Ri质粒的快速提取及rolC基因的克隆  

Rapid Extraction of Ri Plasmid and Cloning of roIC Gene

作　　者：李昌禹[1] 马海琴[1] 赵寿经[1] 臧埔 

机构地区：[1]中国农业科学院特产研究所,吉林吉林132109

出　　处：《特产研究》2003年第2期2-4,8,共4页Special Wild Economic Animal and Plant Research

基　　金：吉林省科学技术厅资助项目(20000554)

摘　　要：介绍Ri质粒的快速提取方法,提取的质粒可用于目的基因的扩增,应用rolC基因的特异性引物,扩增出了正确序列的rolC基因0.87kb片段,并利用pGEM—T载体对此片段进行了克隆。A rapid extraction of Ri plasmid was presented and a 0.87kb fragment of rolC gene was amplified by PCR via primer of rolC gene, the fragment was cloned into vector pGEM-T. The result of sequencing showed that the fragment was exactly rolC gene of T-DNA of Ri plasmid.

关 键 词：RI质粒 快速提取 ROLC基因 基因克隆 pGEM-T载体 

分 类 号：Q782[生物学—分子生物学]