叶子花的组织培养与微繁技术研究  

Studies on tissue culture and micropropogation of Bougainvillea glabra

作　　者：李师翁[1] 范小峰[2] 田兴旺[2] 王毅民[2] 

机构地区：[1]兰州交通大学化工学院,兰州730000 [2]庆阳师范高等专科学校生物系,甘肃西峰745000

出　　处：《西北植物学报》2003年第6期992-996,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：甘肃省教育厅经费资助

摘　　要：叶子花茎尖、茎段接种在MS附加不同浓度组合的6-BA、IAA、IBA、NAA培养基上可诱导茎尖及腋芽的生长而获得无性系芽,无性系芽茎尖和茎段在MS附加6-BA0.5mg/L、NAA0.05mg/L进行继代培养,一般不形成丛生芽,以茎尖生长和腋芽萌生增殖,增殖系数为2.73;高度大于3cm的增殖芽在1/2MS附加IAA1mg/L、IBA1mg/L、NAA0.2mg/L培养基上生根率为80.5%,生根苗用 二步法 移栽成活率在97%以上.无性系芽的幼叶、茎段在MS附加6-BA和NAA、2,4-D培养基上能高频产生愈伤组织,但愈伤组织在所试验的所有培养基上均无不定芽或胚状体的分化.The clonal buds were obtained from shoot apexes and segments of Bougainvillea glabra in vitro by growth of the shoot apexes and axillary buds on MS basic medium supplemented with different concentration of 6BA,IAA,IBA and NAA.The clonal buds were subcultured on MS supplemented with 6BA 0.5 mg/L and NAA 0.05 mg/L,it proliferated with development of the shoot apexes and axillary buds rather than the cluster buds,and the proliferation coefficient is 2.73.The ratio of rooting is 80.5% when more than 3 cm height proliferated buds were cut and transferred onto 1/2 MS supplemented with IAA 1.0 mg/L,IBA 1.0 mg/L and NAA 0.2 mg/L,the ratio of survive is 97% when the little intact plants transplanted on the perlite seedbed.Callus were induced high frequently from the young leaf and shoot segment of clonal buds on MS supplemented with 6BA and NAA,2,4D,but no adventitious buds or embryoid differentiated from the callus on any medium used in testing.

关 键 词：叶子花 组织培养 愈伤组织 微繁 

分 类 号：Q943.1[生物学—植物学]