卡维地洛对高血压大鼠心脏成纤维细胞胶原合成的抑制作用  

The inhibitory effects of carvedilol on collagen syntheysis of cultured cardiac fibroblasts from SHR

作　　者：张海涛[1] 赵连友[1] 王荫静[2] 汪娜 田建伟[1] 王士雯[4] 

机构地区：[1]第四军医大学唐都医院心内科,陕西西安710038 [2]空军总医院临床试验中心,北京100036 [3]北京空军医院特诊科,北京100081 [4]解放军第301医院老年心血管病研究所,北京100853

出　　处：《心脏杂志》2003年第3期205-207,211,共4页Chinese Heart Journal

基　　金：国家重点基础研究发展规划 ( 973)课题资助项目 ( G.2 0 0 0 570 0 4 );陕西省科学技术研究发展计划基金资助项目 ( 2 0 0 0 2 K10 - G 1( 2 3) )

摘　　要：目的 :探讨卡维地洛、比索洛尔、哌唑嗪对培养的 SHR和 Wistar大鼠心脏成纤维细胞 ( CFs)胶原合成的影响。方法 :采用胰酶消化法培养 CFs,用3 H-脯氨酸掺入法分别观察卡维地洛、比索洛尔、哌唑嗪干预下两组大鼠 CFs胶原合成的情况。结果 :1各种浓度的卡维地洛 ( 0 .0 1～ 10μmol/ L )可以浓度依赖的方式抑制 CFs 3 H-脯氨酸掺入量 ,与 Wistar大鼠组相比 ,SHR组 CFs受抑制的效应更强。2同等浓度比索洛尔对两组大鼠 CFs3 H-脯氨酸掺入量表现出轻度抑制。 3同等浓度哌唑嗪对两组大鼠 CFs3 H-脯氨酸掺入量均无明显影响。结论 :卡维地洛呈浓度依赖性抑制 CFs合成胶原 ,其作用机制部分与阻滞β1 受体有关。AIM:To evaluate the effects of carvedilol, bisoprolol and prazosin on collage synthesis of cultured rat cardiac fibroblast(CFs) derived from SHR(CFs SHR ) and Wistar rats (CFs Wis ). METHODS:CFs derived from 12 week old SHR and Wistar rats were cultured with enzymatic dissociation, and collagen synthesis was measured by 3H Proline incorporation after incubation with carvedilol,bisoprolol and prazosin. RESULTS:①Carvedilol(0.01~10 μmol/L)induced a significant concentration dependent decreation in 3H Proline incorporation in CFs derived from SHR and Wistar rats. ②Bisoprolol(0.01~10 μmol/L)caused a slight decrease in 3H Proline incorporation. ③ Prazosin(0.01~10 μmol/L)treament had no effect on the 3H Proline incorporation of all the cells. CONCLUSION:Dose dependent carvedilol inhibites cardiac fibroblasts in vitro ,which is partly due to the property of this selective β 1 adrenergic receptor antagonist.

关 键 词：成纤维细胞 心肌 卡维地洛 比索洛尔 哌唑嗪 胶原合成 

分 类 号：R541.3[医药卫生—心血管疾病]