金银花药材高效液相指纹图谱研究  

Study on Fingerprint of Honeysuckle by High Performance Liquid Chromatography

作　　者：刘艳娥[1] 吴玫涵 吴玉田[2] 曹红 

机构地区：[1]中国人民解放军总后勤部卫生部药品仪器检验所,北京100071 [2]中国人民解放军第二军医大学药学院药物分析教研室,上海200433

出　　处：《解放军药学学报》2003年第3期161-164,共4页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：全军"十五"课题No .0 1Z0 64

摘　　要：目的　建立可供鉴别金银花药材的高效液相 (HPLC)指纹图谱。方法　采用高效液相色谱梯度洗脱法 ,选用ZorbaxSB-C18(5 μm ,4 .6× 2 5 0mm)色谱柱 ;流动相 :(A)甲醇 - 0 .0 5 %磷酸溶液 (10∶90 ) ,(B)甲醇 - 0 .0 5 %磷酸溶液 (6 0∶4 0 )梯度洗脱 ,采用线性梯度洗脱程序 ;流速 :1ml/min ,运行时间 6 0min。检测波长为 2 38nm。结果　确定了金银花药材有 11个共有峰。结论　方法可靠 ,为提高金银花药材质量控制标准提供参考。Aim To establish fingerprint chromatography of Traditional Chinese Medicine honeysuckle by HPLC.Method The HPLC method was used,chromatography conditions were Zorbax SB-C 18 column,methanol with 0.05% phosphoric acid as mobile phase and 238nm at detective wavelength.Results The result showed that 11peaks were common.Conclusion The method is reliable and provides a reference standard for the quality control of honeysuckle.

关 键 词：金银花 中药材 高效液相色谱法 指纹图谱 质量控制 化学成分 

分 类 号：R282.71[医药卫生—中药学]