半连续培养JAL1杂交瘤细胞的生长和代谢  

Growth and Metabolism of JALl Hybridoma Cells in Semicontinuous Cultures

作　　者：赵渝[1] 赵冰[2] 胡昕[2] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]上海交通大学农学院,上海201101

出　　处：《上海师范大学学报（自然科学版）》2003年第2期64-67,共4页Journal of Shanghai Normal University(Natural Sciences)

基　　金：上海市教委基金(98E05)

摘　　要：观察了分泌抗IBDV单抗杂交瘤细胞在改良型5L Celligen细胞反应器中采用半连续培养方式的生长和代谢情况,研究了在该工艺过程中的葡萄糖消耗、单抗的生成、乳酸和氨的产生。同时为培养方案和控制策略进一步的改良确定方向。The growth and metabolism of JAL1 hybridoma cells were studied in semicontinuous cultures in improved Celligen bio-reactor (5L).The utilization of glucose and the production of antibody,lactic acid and ammonia were also investigated.Meanwhile,the discussion of operation mode and control strategy in bioreactor culture indicates the direction of their improvement.

关 键 词：IBDV 杂交瘤 半连续培养 

分 类 号：Q953.31[生物学—动物学]