高效液相色谱法测定火麻仁油中Δ~9-四氢大麻酚的含量  

Determination of Δ~9-tetrehydrocannabinol in the hempseed oil by HPLC

作　　者：张岗[1] 郭江宁[1] 毕开顺[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2003年第4期269-271,共3页Journal of Shenyang Pharmaceutical University

摘　　要：目的建立火麻仁油中Δ9 四氢大麻酚的含量测定方法。方法采用Irregular H C18(2 5 0mm× 4 6mm ,10 μm)为色谱柱 ,以甲醇 乙腈 水 (10∶6 5∶2 5 ,V∶V∶V)为流动相的高效液相色谱法 ,流速 1 0mL·min-1,检测波长 2 2 0nm ,柱温室温。结果Δ9 四氢大麻酚在 1 2～9 6mg·L-1内呈良好的线性关系 (r =0 9998) ;样品平均加样回收率为 96 2 % ,RSD为 2 1%(n =9)。结论方法简便快速 ,适用于火麻仁油中Δ9Objective To establish the analytical method for Δ 9 tetrahydrocannabinol in the hempseed oil.Methods The content of Δ 9 tetrahydrocannabinol was determined by HPLC.Irregular H C 18 ( 250 mm × 4 6 mm , 10 μm )was used.The mobile phase consisted of methanol,acetonitrile and water (10∶65∶25, V∶V∶V) .The flow rate was 1 0 mL·min -1 and the UV detector was set at 220 nm .The column temperature was room temperature.Results The calibration curve was good linear ( r =0 999 8 ) within the concentration range of 1 2～ 9 6 mg·L -1 for Δ 9 tetrahydrocannabinol.The average recovery and RSD were 96 2% and 2 1%( n =9) respectively.Conclusions The method is convenient,fast and suitable for the quality control of the Δ 9 tetrahydrocannabinol in the hempseed oil.

关 键 词：火麻仁油 △^9-四氢大麻酚 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]