天津野生鲫鱼卵黄蛋白原的研究  

Investigation of vitellogenin in wild crucian (Carassius auratus) Tianjin.

作　　者：胡建英[1] 安立会[1] 孙晓航[1] 张海峰[1] 邓宝山[1] 

机构地区：[1]北京大学环境学院,北京100871

出　　处：《中国环境科学》2003年第3期281-284,共4页China Environmental Science

基　　金：国家杰出青年科学基金资助项目(49925103);环保"863"(2001AA646010-5)

摘　　要：用HPLC方法提纯经雌二醇诱导的鲫鱼卵黄蛋白原,用SDS-PAGE凝胶电泳测定其分子量为160Kda左右.在建立卵黄蛋白原(VTG)的HPLC分析方法(检测下限为0.025mg/mL)的基础上,对北京排污河天津段的野生和养殖鲫鱼血浆中的VTG进行检测.在所捕获的12尾野生鲫鱼中(其中2尾为雄性鲫鱼),都检测出了VTG,浓度范围为0.284～5.971mg/mL;对同期捕获的35尾天津养殖鲫鱼包括1尾雄性鲫鱼中的VTG也进行了检测,浓度范围为0.119～0.250mg/mL.而同期在实验室饲养的雌雄鲫鱼血浆中,均未检测出VTG.结果表明,北京排污河(天津段)的野生鲫鱼受到类雌激素物质的污染.Vitellogenin (VTG) in crucian (Carassius auratus) induced by injection of 17?estradiol was purified using HPLC method, and its molecular weight was determined to be about 160Kda using SDS-PAGE. Based on the HPLC analysis method of vitellogenin established (its low limit of detection was 0.025靏/mL), the VTG in the plasma of wild and cultured crucians from Beijingpaiwu River in Tianjin area was detected. Vitellogenin was detected in all of 12 caught wild fishes, (including 2 male fishes) and the concentration ranged from 0.284 to 5.971mg/mL. Vitellogenin was also detected in all of 35 cultured fishes including 1 male, catched in same period and the concentration ranged from 0.119 to 0.250mg/mL; but no vitellogenin was detected in both male and female cultured in laboratory in same period. The wild crucian from Beijingpaiwu River in Tianjin was contaminated by estrogenic matter.

关 键 词：内分泌干扰物 鲫鱼 卵黄蛋白原 生物标志物 

分 类 号：X703.5[环境科学与工程—环境工程]