脂联素基因Gly15Gly多态性与2型糖尿病的相关性研究  

Study on association of Gly15Gly polymorphism in adiponectin gene with type 2 diabetes

作　　者：靳立忠[1] 刘德敏[1] 于德民[1] 李戈[1] 汪玮琳[1] 范新萍[1] 

机构地区：[1]天津医科大学代谢病医院,天津300070

出　　处：《天津医科大学学报》2003年第2期211-213,共3页Journal of Tianjin Medical University

摘　　要：目的 :研究脂联素基因外显子2Gly15Gly多态性与2型糖尿病的相关关系。方法 :采用聚合酶链反应 -限制性片段长度多态法 (PCR -RFLP)检测122名正常对照者和92名2型糖尿病患者脂联素基因外显子2Gly15Gly基因型 ,比较两组基因型和等位基因频率。结果 :对照组122例中TT、TG、GG基因型分别为64(52.5 % )例、56(45.9% )例、2(1.6% )例 ,糖尿病组92例中TT、TG、GG基因型分别为52(56.5% )例、32(34.8% )例、8(8.7% )例 ,糖尿病组GG基因型频率高于对照组 ( χ2=5 86 ,P<0.05),两组等位基因频率未见显著性差异(χ2=0.12,P>0.05)。结论 :脂联素基因外显子2Gly15Gly 多态性与2型糖尿病相关 。Objective: To study the inter relationship between Gly15Gly polymorphism in adiponectin gene exon 2 with type 2 diabetes.Methods :In 122 healthy controls and 92 patients with type 2 diabetes, to analyze the Gly15Gly genotypes of adiponectin gene exon 2 by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP), and compare the frequencies of genotype and allele in two groups.Results:In 122 healthy controls the TT, TG, and GG genotypes were found in 64(52.5%),56(45.9%) and 2(1.6%) cases, and in 92 patients with type 2 diabetes the TT, TG, and GG genotypes were found in 52(56.5%), 32(34.8%)and 8(8.7%) cases respectively, the GG genotype frequency of type 2 diabetes patients was higher than that of the healthy controls(χ2=5.86,P<0.05),the allelic frequency between two groups showed no significant difference(χ2=0.12,P>0.05).Conclusion:The Gly5Gly polymorphism in adiponectin gene exon 2 is associated with type 2 diabetes, the people with GG genotype have high susceptibility of type 2 diabetes.

关 键 词：脂联素 2型糖尿病 限制性片段长度多态性 

分 类 号：Q75[生物学—分子生物学]