高脂大鼠抵抗素基因的上调表达及与胰岛素、瘦素的相关性  

Resistin gene overexpression in adipose tissue of SD rats with high-fat diet and its correlation with leptin and insulin

作　　者：王绍云[1,2] 宋怀东[1,2] 李荣英[1,2] 唐金凤[1,2] 李凤英[1,2] 周丽斌[1,2] 杨颖[1,2] 胡仁明[1,2] 牟波[1,2] 陈名道[1,2] 

机构地区：[1]上海第二医科大学附届瑞金医院内分泌代谢科 [2]上海市内分泌代谢病研究所

出　　处：《中华内分泌代谢杂志》2003年第3期191-193,共3页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金资助项目(30270626)

摘　　要：目的 观察SD大鼠高脂饮食状态下脂肪组织中抵抗素的表达及其与胰岛素抵抗以及糖尿病、瘦素等的关系。方法 采用RT-PCR、割胶纯化、基因构建测序的方法,证实为目的片段后,对高脂饮食SD大鼠的脂肪组织进行mRNA抽提,再以此为模板进行半定量RT-PCR扩增及Northern印迹分析。同时检测血清中胰岛素、瘦素水平,并行糖耐量试验、胰岛素释放试验等。结果 (1)成功构建了抵抗素的基因,并经测序证实。(2)与对照组相比,高脂饮食组大鼠脂肪组织中抵抗素表达明显升高,血糖水平、胰岛素水平以及瘦素水平升高。(3)高脂饮食组大鼠在4个月时下丘脑中瘦素受体表达较对照组明显降低。结论 抵抗素表达量的变化与饮食有关,抵抗素可能是胰岛素抵抗、糖尿病发病的一个前期信号,其作用可能与瘦素、瘦素受体有一定关系。Objective To observe expression of resistin gene in adipose tissue of SD rats fed with high-fat diet and its correlation to insulin resistance, diabetes mellitus and leptin. Methods Using RT-PCR to mRNA from adipose tissue, the products were obtained, purified, constructed and squenced. Once its squence was verified, semi-quantitative RT-PCR and Northern Blot were employed to study its expressions in adipose tissues of rats with different diets. Oral glucose tolerance test and insulin release test were performed in 2 groups of rats. Results (1) The resistin gene was successfully constructed and sequenced, which was in accordance with the data of genbank. (2) Compared with the control, the expression of resistin in adipose tissue of high-fat diet rats was significantly enhanced, and blood glucose, serum insulin and leptin levels were also elevated in high-fat diet rats (P<0.05). (3) The expression of leptin-receptor in hypothalamus tissue of high-fat diet rats was reduced as compared with normal diet rats. Conclusion Resistin is expressed in adipose tissue of rats and the expression level varied in different diet rats, being up-regulated in rats with high-fat diet. Resistin may act as an antecedent singal of insulin resistance as well as diabetes mellitus, and its effect seems to be related, at least partially, to leptin and leptin receptor.

关 键 词：肥胖 糖尿病 脂肪组织 抵抗素 胰岛素抵抗 胰岛素 瘦素 基因表达 糖尿病 

分 类 号：R58[医药卫生—内分泌]