黄芪总多糖对豚鼠皮肤HSV-1 HS-1株感染的治疗作用  

The Therapeutic Effects on Human Simplex Virus Cutaneous Infection of Guniea Pigs of Total Polysaccharides in the Root of Astragalus Membranaceus

作　　者：王志洁[1] 黄铁牛[2] 刘建军[1] 董长垣[1] 

机构地区：[1]武汉大学医学院病毒研究所,湖北武汉430071 [2]中南财经政法大学

出　　处：《成都中医药大学学报》2003年第2期35-37,共3页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：湖北省自然科学基金课题 (编号 :980J0 78)

摘　　要：目的　观察黄芪总多糖在活体中的抗人疱疹病毒I型的作用。方法　以阿昔洛韦 (ACV)为阳性对照 ,以磷酸缓冲的盐水 (phosphatebufferedsaline ,PBS)以及含 10 %DMSO (二甲亚砜 )的PBS为阴性对照 ,用HSV - 1HS - 1株感染豚鼠皮肤的动物模型 ,观察了黄芪总多糖抗HSV - 1药效。结果　ACV、黄芪总多糖处理皮区的累积计分分别为 14 2 0± 6 70、 12 75± 3 5 8。ACV、黄芪总多糖处理皮区的痊愈天数分别为 7 70± 1 0 8、 8 5 0± 0 5 0 ;与PBS及含 10 %DMSO的PBS处理皮区之差异 ,有统计学意义 (P <0 0 5 )。HSV - 1HS - 1株感染过程中 ,各处理皮区的大体照片及皮肤样本的病毒滴度检测 ,获得了一致的结果。ACV与黄芪总多糖处理皮区的累积计分、痊愈天数 ,以及两种方法处理皮区皮肤样本的病毒检测结果 ,其差异均无统计学意义 (P >0 0 5 )。结论　黄芪总多糖与ACV药效相近 ,值得开发利用。Objective:To investigate the antiherpes effects on human simplex v irus type-1 of total polysaccharides of Astragalus membranaceus in vivo Methods:Used Acyclovir as positive control ,and used PBS and PBS which contained 10%DMSO as negative control ,used guniea pigs model of cutaneous herpes virus infection,observed the antiherpes effects of total polysaccharides in Astragalus membranaceus Results:The results of clinical observations showed that the cumulative score of the regions dealt with ACV,total polysaccharides were 14 20±6 70, 12 75±3 58 respectively; the time to healing days of the regions dealt with ACV,total polysaccharides were 7 70±1 08, 8 50±0 50 separately,these results had significances in statistics when they were compared with the regions dealt with PBS and PBS which contained 10%DMSO The pictures of each treatment regions and the measutements of virus titres of skin samples got same results Conclusion:It was suggested that the cumulative scores and time to healing days of skin regions dealt with ACV had no differences in statistics with the cumulative scores and time to healing days of akin regionsdealt with total polysaccharides,these results added up the results of measurement of virus titre and the pictures of the regions dealt with each drug to suggest the antiherpes effects of the total polysaccharides were close to ACV,and should be worth exploitation

关 键 词：黄芪总多糖 豚鼠 皮肤 HSV—1HS—l株感染 治疗作用 

分 类 号：R285.5[医药卫生—中药学]