几种黄连解毒汤的HPLC指纹图谱研究  

Studies on HPLC Fingerprint of several kinds of Huanglianjiedu Decoction

作　　者：沈嘉[1] 曹现峰[2] 刘九飞[3] 

机构地区：[1]沈阳药科大学,辽宁沈阳110015 [2]江苏恒瑞医药股份有限公司,江苏连云港222002 [3]锦州医学院药学院

出　　处：《中成药》2003年第6期433-437,共5页Chinese Traditional Patent Medicine

基　　金：辽宁省教育厅资助项目 (N .2 0 15 2 2 2 8)

摘　　要：目的 :以HPLC指纹图谱区分不同工艺、不同原料制备的黄连解毒汤 (黄连、黄芩、黄柏、栀子 )制品及日本汉方黄连解毒汤制剂。方法 :HPLC法 ;采用C18V反相色谱柱 ,二元梯度洗脱 ,二极管阵列检测器 ,以芍药苷为内标作为相对标准。结果 :共有 15～ 16个特征峰为共有峰 ,但新工艺制品和醇提物出现 1个独特的特征峰 ,与其它制品不同。各不同工艺及原料制品的特征峰与内标峰的峰高比有显著性区别。结论 :试验结果稳定 ,重复性良好 。Objective:To identify Huanglianjiedu Decoction preparation (Rhizoma Coptidis, Radix Scutellariae, Cortex Phellodendri, Fructus Gardeniae) made from different technics and Japanese Kampo with fingerprint chromatographic. Methods: The HPLC method was used, chromatography conditions were C 18V column, binary solvent-delivery gradient elution, diode-array detector, internal standard (paeoniflorin) were taken as the criteria for quality control. Results: The result showed that 15～16 characteristic peaks were common, but the new technic preparation and EtOH extract showed a specific characteristic peak. And significant deviation has been found from peak-height ratio of characteristic peaks to internal standard in various preparations, which were made from different technics or from different materials. Conclusion: The good reproducibility and stability are showed in experiment results. The HPLC fingerprint can be used for identifying this traditional Chinese medicine preparations which were made from different technics or from different materials.

关 键 词：黄连解毒汤 指纹图谱 高效液相色谱 日本汉方 

分 类 号：R289.5[医药卫生—方剂学] R927.2[医药卫生—中药学]