抗人钠碘转运体单克隆抗体杂交瘤细胞系的建立与初步鉴定  

The development and characterization of monoclonal antibody against human sodium iodide symporter

作　　者：袁庆新[1] 刘超[1] 段宇[1] 仇镇宁[1] 管晓红[1] 

机构地区：[1]南京医科大学第一附属医院,210029

出　　处：《江苏医药》2003年第6期419-421,共3页Jiangsu Medical Journal

基　　金：国家自然科学基金资助项目 (3 9970 3 5 1)

摘　　要：目的　制备抗人钠碘转运体 (NIS)单克隆抗体。方法　以人NIS蛋白质的二个片段(第 2膜外段和第 14f段 )为抗原免疫小鼠 ,运用杂交瘤技术 ,制备鼠抗人NIS单克隆抗体 (rhNISMAb) ,采用小鼠Ig亚类测定试剂条测定单抗的亚类 ,并通过ELISA、Western blot、免疫组化技术等鉴定其特异性。结果　运用杂交瘤技术成功地制备出能稳定分泌抗人NIS单克隆抗体的细胞系。Western blot分析表明 :此单抗所识别的靶分子的相对分子量主要在 97KD ,免疫组化染色显示 :甲状腺滤泡细胞基底膜上有棕黄色着色。结论　成功地制备出抗人NIS单克隆抗体 ,为NIS抗原 抗体的研究提供了新的工具 ,NIS单克隆抗体不仅可以应用于甲状腺、乳腺等领域的基础研究 ,而且可以用于临床 。Objective To develop monoclonal antibody against human sodium iodide symporter.Methods The hybridoma cell lines were obtained by fusing spleen cells of Balb/c mice that had been immunized with synthesized NIS peptides.The monoclonal antibody was characterized by ELISA,Western-blot and immunohistochemical staining.Results One hybridoma cell line was developed.This cell line could stably secrete monoclonal antibody which could react specifically to synthesized NIS peptides.The result of Western-blot showed that the monoclonal antibody could recognize molecules of relative molecular mass 97KD in the target cells.Immunostaining with this monoclonal antibody was observed in Graves thyroid tissues.Conclusion Monoclonal antibody to human sodium iodide symporter has been developed and characterized successfully.

关 键 词：人钠碘转运体 单克隆抗体 杂交瘤技术 电泳印渍检测法 

分 类 号：R392.11[医药卫生—免疫学]