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作 者:ShenXin ThomasTeichmenn WangYiqin BaiGenben YuGuangjun WangShasheng
机构地区:[1]CollegeofBiologicalSciencesandBiotechnology,BeijingForestryUniversity,Beijing100083,P.R.China [2]ForestbotanischesInstitut,UniversityGoettingen,Buegenweg2,37077,Goettingen,Germany [3]GeneticsInstitute,ChineseAcademyofSciences,Beijing100010,P.R.China
出 处:《Forestry Studies in China》2003年第1期8-12,共5页中国林学(英文版)
基 金:National Natural Science Foundation of China(Grant No.39830320)
摘 要:Through construction of a subtracted cDNA library and library screening, a number of salt-induced cDNA fragments have been cloned from Populus euphratica. Based on the results of DNA sequencing and Northern analysis, the gene response of Populus euphratica to salt stress is discussed. It is indicated that in response to salt treatment the transcription level for some genes of Populus euphratica increases by about 1.5 times and significant difference between the responses to osmotic stress and to ion stress has been observed in gene activity.Through construction of a subtracted cDNA library and library screening, a number of salt-induced cDNA fragments have been cloned from Populus euphratica. Based on the results of DNA sequencing and Northern analysis, the gene response of Populus euphratica to salt stress is discussed. It is indicated that in response to salt treatment the transcription level for some genes of Populus euphratica increases by about 1.5 times and significant difference between the responses to osmotic stress and to ion stress has been observed in gene activity.
关 键 词:Populus euphratica cDNA subtraction differentially expressed gene salt-tolerance mechanism
分 类 号:S792.11[农业科学—林木遗传育种]
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