IL-12对结构优化的HBV核心抗原DNA疫苗诱导免疫应答的影响  

Impact to immune responses by interleukin 12 on optimized hepatitis B core antigen DNA vaccine in mice

作　　者：高军[1] 赵平[1] 柯金山[2] 戚中田[1] 

机构地区：[1]第二军医大学微生物学教研室 [2]第二军医大学长征医院实验诊断科

出　　处：《中华微生物学和免疫学杂志》2003年第5期355-358,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金 ( 3 9980 0 3 8) ;上海科技发展基金( 0 0 43 192 0 6)资助项目

摘　　要：目的　探讨IL 12对一种结构优化的HBV核心抗原 (HBcAg)DNA疫苗免疫效果的影响。方法　将小鼠IL 12基因插入结构优化的DNA疫苗pST HBc,构成pST HBc/IL12 ,将pST HBc/IL12转染COS7细胞 ,ELISA检测培养上清中的HBcAg和小鼠IL 12。分别将pST HBc/IL12和pST HBc肌肉注射接种BALB/c小鼠 ,检测小鼠的体液和细胞免疫应答。结果　ELISA检测到培养上清液中的HBcAg和小鼠IL 12 ,pST HBc/IL12诱导的抗 HBc总IgG阳转率和抗体水平不及pST HBc ,但其诱导的脾细胞增殖反应和细胞毒性T淋巴细胞反应均强于pST HBc。结论　IL 12可进一步增强这种HBcAgDNA疫苗诱导的细胞免疫应答。Objective To observe the influence of immune responses stimulated with interleukin 12 (IL 12) on optimized hepatitis B core antigen (HBcAg) DNA vaccine. Methods Murine IL 12 cDNA was inserted into optimized HBcAg DNA vaccine pST HBc. The resultant recombinant expression plasmid pST HBc/IL12 was transfected into COS7 cells and its expressed product was detected by ELISA. BALB/c mice were immunized intramuscularly with pST HBc, pST HBc/IL12 or mock vector, respectively. The humoral and cellular immune responses of the mice were detected. Results pST HBc/IL12 could express secretory HBcAg and murine IL 12. In mice, pST HBc/IL12 induced weaker anti HBc IgG but stronger T lymphocyte proliferative response and cytotoxic T lymphocyte response than that of pST HBc did. Conclusion IL 12 enhanced cellular immune responses that were elicited by optimized HBcAg DNA vaccine. This result may be refered in the development of therapeutic vaccine against hepatitis B. [

关 键 词：乙型肝炎病毒 DNA疫苗 HBV核心抗原 白细胞介素-12 

分 类 号：R392[医药卫生—免疫学]