聚天冬氨酸硅质固定相的研制及应用  

Synthesis and Application of Poly(D,L-aspartic Acid)-Silica Stationary Phase

作　　者：刘淑娟[1] 祝馨怡[1] 周文峰[1] 刘霞[1] 蒋生祥[1] 

机构地区：[1]中国科学院兰州化学物理研究所,甘肃兰州730000

出　　处：《分析测试技术与仪器》2003年第2期80-83,共4页Analysis and Testing Technology and Instruments

摘　　要：D,L 天冬氨酸在浓磷酸的存在下加热聚合生成聚琥珀酰亚胺,此产物能与氨丙基硅胶快速反应生成聚琥珀酰亚胺硅胶,然后再水解生成聚天冬氨酸硅质固定相.并对反应条件进行了优化.实验表明,聚天冬氨酸固定相对蛋白质有较好的分离能力和选择性.In the presence of concentrate H\-3PO\-4 the poly(succinimide) was quantitatively polymerized by heating D,Laspartic acid. The product quickly reacted with aminopropylsilica, and then was hydrolyzed to poly(aspartic acid)silica. It was found that the poly(aspartic acid)silica stationary phase had good ability to separate proteins. The reaction conditions were also optimized during the investigation.

关 键 词：蛋白质 分离技术 聚天冬氨酸 固定相 D L-天冬氨酸 聚琥珀酰胺硅胶 研制 应用 

分 类 号：Q51[生物学—生物化学] O633.5[理学—高分子化学]