11种软体动物粗蛋白对微生物细胞的凝集性能初探  

Study on the Agglutination of crude proteins from 11 Species of Mollusca to the Microbial Cells

作　　者：张鹭[1] 陈寅山[1] 谢华玲[1] 

机构地区：[1]福建师范大学生物工程学院

出　　处：《中国海洋药物》2003年第3期33-36,共4页Chinese Journal of Marine Drugs

基　　金：福建省教育厅资助项目(A类)(项目编号:JA02179)

摘　　要：选11种福建常见的软体动物粗蛋白,检测其对细菌、酵母菌、霉菌孢子等7种微生物单细胞的凝集活性,凝集素检出率达82％。啤酒酵母菌的菌体(包括芽体)对凝集民应最为敏感。凝集素有较高的温度耐受性,其中5种凝集素均能耐受75℃以上15min的高温处理。但对酸性环境极为敏感,pH<6.5时即无法检出凝集活性。凝集反应可以被0.02mol·L^(-1)EDTA所抑制,也可以被甘露糖、葡萄糖、果糖、半乳糖抑制。9种糖中山梨醇的抑制专一性最强,仅能抑制杂色地的凝集活性,最大抑制浓度为150mg·L^(-1)。11 species of Fujian common Mollusca were selected to abstract crude proteins and to detect their agglutinating activity with seven kinds of single-cell microbe including Bacteria、Saccharomyces carlsbergensis、mold spores. agglutinin-selected rate was 82% and S.carlsbergensis was more sensitive to agglutination than other cells. The agglutinins have a high stability to haet and all five agglutinins can tolerate for 15 min in 75℃, but they showed a higher sensitivity to acid conditions. No agglutination was observed when pH was under6.5. Agglutination with S. carlsbergensis can be inhibited by EDTA 0. 02mol·L-1 as well as D-glucose、D-fructose、D-mannose and D-galactose. Among nine kinds of saccharides,the agglutination-inhibiting ability of D-sorbitol has the highest particularity, because D-sorbitol can only inhibite the agglutinating activity of 150mg · L-1 Haliotis diversicolor Reeve.

关 键 词：软体动物 粗蛋白 微生物 细胞 凝集性能 凝集素 

分 类 号：Q503[生物学—生物化学]