纳洛酮减弱白细胞介素-2对大鼠心肌的抑制作用  

Naloxone for attenuation of interleukin-2-induced myocardial depression in rat hearts

作　　者：屠洁[1] 胡爱萍[1] 曹春梅[1] 夏强[1] 

机构地区：[1]浙江大学医学院生理学教研室,浙江杭州310031

出　　处：《浙江大学学报（医学版）》2003年第3期192-196,共5页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省自然科学基金青年人才专项资金 [RC990 38]

摘　　要：目的 :研究白细胞介素 - 2 (IL - 2 )的心脏作用并探讨其相关机制。方法 :采用视频跟踪系统测定大鼠单个心室肌细胞的收缩 ;细胞内双波长钙荧光系统检测细胞内游离钙离子浓度 ;应用 Langendorff灌流装置 ,观察 IL - 2对完整心脏收缩力学的影响。结果 :与对照相比 ,IL - 2 (5、5 0 U/ml)显著抑制单个心室肌细胞的收缩幅度 [(74 .95±4 .79vs98.0 9± 5 .0 2 ) % ,(6 4.30± 5 .2 4 vs97.38± 4 .0 5 ) % ]、最大收缩速度 [(70 .2 3± 4 .85 vs98.0 9± 5 .4 6 ) % ,(6 1.15± 5 .2 0 vs97.38± 6 .85 ) % ]、最大舒张速度 [(71.2 2± 4 .79vs98.32± 6 .0 8) % ,(6 8.16± 5 .2 4 vs97.5 5±5 .0 0 ) % ]和舒张末细胞长度 [(88.2 8± 5 .84 vs97.95± 5 .5 2 ) % ,(84 .18± 6 .5 2 vs98.94± 6 .76 ) % ];IL- 2 (5、5 0 U/ml)显著降低电刺激诱导的心肌细胞内钙瞬变幅度 [(74 .94± 4 .90 vs98.0 9± 3.74 ) % ,(71.0 0± 5 .2 8vs97.38±5 .5 2 ) % ],使舒张末钙水平升高 [(113.91± 5 .93vs10 0 .10± 3.0 2 ) % ,(119.0 9± 7.12 vs10 0 .5 2± 6 .0 0 ) % ]。阿片受体阻断剂纳洛酮 (10 nmol/L)可阻断 IL- 2对心肌细胞收缩和细胞内钙的作用。在离体心脏 ,与对照相比 ,IL- 2 (5 0U/ml)Objective: To investigate the cardiac effect of interleukin-2 (IL-2) and to explore the underlying mechanism.Methods:The video tracking system and spectrofluorometric method were used to measure the cell contraction and intracellular calcium.Fura-2/AM was used as a calcium fluorescence probe.Langendorff perfusion technique was used to determine the effect of IL-2 on the intact heart.Results:Compared with the control group,IL-2(5 U/ml,50 U/ml)significantly decreased cell contraction amplitude [(74.95±4.79) vs (98.09±5.02)%,(64.30±5.24) vs ( 97.38± 4.05)%],peak velocity of cell shortening [(70.23±4.85)% vs (98.09±5.46)%,(61.15±5.20)% vs (97.38±6.85)%],peak velocity of cell relengthening [(71.22±4.79)% vs (98.32± 6.08)%,(68.16±5.24)% vs (97.55±5.00)%] and end-diastolic cell length [(88.28±5.84)% vs (97.95± 5.52)%,(84.18± 6.52)% vs (98.94±6.76)%].IL-2 (5 U/ml,50 U/ml) also markedly inhibited intracellular calcium transient [( 74.94±4.90)% vs (98.09±3.74)%,(71.00±5.28)% vs (97.38±5.52)%],and elevated end-diastolic calcium level of ventricular myocytes [(113.91±5.93)% vs (100.10±3.02)%,(119.09±7.12)% vs (100.52±6.00)%],which were attenuated by the opioid receptor antagonist naloxone (Nal,10 nmol/L).In the isolated perfused rat heart,when compared with the control group,IL-2(50 U/ml)markedly decreased left ventricular developed pressure [(79.91±2.18) vs (93.84±2.94)mmHg],maximal rate of rise of left ventricular pressure [(2370.73± 58.29) vs (2591.50±62.81)mmHg] maximal rate of fall of left ventricular [-(1460.95±38.6) vs -(1634.24± 54.05)mmHg/s] and heart rate [(217.35±10.56) vs (244.52±11.23)beats/min],but increased left ventricular end-diastolic pressure (11.44±1.02 vs 9.23±0.46).Pretreatment with Nal (10 nmol/L) antagonized the cardiac depression and left ventricular end-diastolic pressure elevation induced by IL-2.Conclusion:The cardiac effect of IL-2 is mediated by opioid receptors on the membrane of cardiomyocytes.

关 键 词：纳洛酮 白细胞介秦-2 大鼠 心室肌细胞 抑制作用 视频跟踪系统 测定 细胞内游离钙离子浓度 

分 类 号：R541[医药卫生—心血管疾病]