创伤性颅脑损伤后细胞凋亡状态及相关基因表达的实验研究  

Cell apoptosis and the expression of bcl-2and bax genes in neurons after traumatic brain injury in rats

作　　者：杨小锋[1] 朱永坚[1] 刘伟国[1] 吕世亭[1] 

机构地区：[1]浙江大学医学院附属第二医院,杭州310009

出　　处：《浙江医学》2003年第6期321-323,共3页Zhejiang Medical Journal

基　　金：浙江省医学科研基金资助项目 (2000A110)

摘　　要：目的 探讨不同程度颅脑损伤后bcl-2、bax基因表达与细胞凋亡的关系。方法 将100只SD大鼠分为轻、中、重度损伤组和对照组 ,通过改进的Feeney自由落体损伤装置制作脑损伤模型 ,然后用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法以及免疫组织化学方法 ,对各组大鼠脑组织中的凋亡细胞和bcl-2、bax免疫反应阳性细胞进行检测和统计。 结果 各损伤组大鼠脑组织中凋亡细胞标记指数 (LI)明显高于未损伤的对照组(均P<0.05) ,bcl-2、bax阳性细胞LI也有所增加 (均P<0.05) ,同时 ,各损伤组bax/bcl-2阳性细胞LI的比值均大于对照组 (均P<0.05) ;轻度、中度组与重度组之间各项指标的差别大多有显著性意义 ,损伤程度重者指标升高明显。结论 bcl-2和bax表达的比例对颅脑损伤后促进细胞凋亡可能起重要的调控作用 ,其表达水平与损伤程度成正比。Objective To investigate the relationship between the expression of bcl-2,bax genes and posttraumatic cellular apoptosis in various degrees of head injury in rats.Methods In the study the improved Feeney's free falling body injury model was adopted in100rats.The cell apoptosis was examined with TUNEL(terminal-deoxynucleotidyl transferase mediated nick end labeling)method and the bcl-2and bax gene exˉpression was detected with Immunohistochemisty method.Results The apoptosis Labeling Index(LI)was sigˉnificantly higher in injured groups than that in controls(P<0.05)and the expression rate of bcl-2and Bax was also higher in the injured rats(P<0.05).And in injured groups the ratio of bax/bcl-2was higher than that in control group(P<0.05).These parameters in mild and moderate groups presented significant differences compared with the severe group.Conclusion The expression of bcl-2and bax may play an important role in posttraumatic cellular apoptosis of brain and the levels of bcl-2and bax were proportional to the degree of head injury.

关 键 词：创伤性 颅脑损伤 细胞凋亡 相关基因表达 实验研究 

分 类 号：R651.15[医药卫生—外科学]