桥本甲状腺炎甲状腺组织细胞凋亡与凋亡基因Bc1-2、Bax表达  

The Studies on Thyrocyte Apoptosis and Expression of Bcl-2 and Bax in Hashimoto′s Thyroiditis

作　　者：赵亚平[1] 王加林[1] 范智勇[1] 刘泽红[1] 吴合军[1] 周玮[1] 贾美斋 

机构地区：[1]解放军第82医院内分泌专科中心,江苏淮安223001

出　　处：《标记免疫分析与临床》2003年第2期70-73,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：为了探讨桥本甲状腺炎 (HT)甲状腺组织细胞凋亡的变化、凋亡基因Bc1- 2、Bax的表达及其与HT发病机制的关系 ,采用TUNEL法检测 4 1例HT、10例正常甲状腺组织细胞凋亡状况 ,同时采用免疫组化S -P法检测Bc1- 2、Bax表达及分布 ,应用Mias99图像分析系统对免疫组化结果进行定量分析。结果表明 :HT组甲状腺细胞凋亡率及甲状腺组织Bc1- 2、Bax阳性颗粒面积、平均光密度和积分光密度均显著高于正常组 (P <0 .0 5 - 0 .0 0 1) ;HT甲状腺滤泡区Bc1- 2阳性颗粒面积和积分光密度与Bax的比值明显低于正常甲状腺的比值 ,其组织结构破坏较重区域中小滤泡及失去正常滤泡结构的腺上皮Bax表达高于其它腺上皮 ,滤泡结构较完整的区域Bc1- 2表达较高 ,提示甲状腺细胞凋亡增高是HT甲状腺滤泡结构破坏和功能异常的重要机制之一 ;凋亡基因Bc1- 2。To investigate the thyrocyte apoptosis,the expression of Bcl-2,Bax and the relationship between apoptosis and the pathogenesis in Hashimoto′s thyroiditis(HT),41 HT thyroid and 10 normal thyroid specimens were selected. The level of apoptosis was detected by TUNEL methods. The expression and distribution of Bcl-2 and Bax were detected using immunohistochemical methods and analyzed by Mias99 pathological image system.Immunohistochemical staining was carried out using S-P kit.The Result showed that an increased level of apoptosis was observed in Hashimoto′s glands.The apoptosis mainly distributed in thyroid follicles destruction area.This was associated with increased Bax expression.The strongly positive Bcl-2 staining was observed in the thyrocyte of intact thyroid follicles.The ratios of positive granule area and total light density of Bcl-2 to those of Bax in HT thyroid follicle area were lower than those in normal thyroid.The apoptosis of thyrocyte induced by dysregulation of Bcl-2 and Bax may be involved in the pathogeneses of HT.

关 键 词：桥本甲状腺炎 甲状腺组织 细胞凋亡 凋亡基因 Bcl-2 Bax 表达 

分 类 号：R581.4[医药卫生—内分泌]