生物活性陶瓷材料对血浆蛋白吸附及钙、磷影响的实验研究  

Experimental Study of Plasma Protein Adsorption and Effect of Ca and P on Bioactive Ceramics for Artificial Bone

作　　者：孙涛[1] 朱明华[1] 曾怡[1] 彭蔷[1] 叶长宁[2] 

机构地区：[1]四川省生物医学材料监测中心,四川成都610041 [2]四川省人民医院检验中心,四川成都610072

出　　处：《透析与人工器官》2003年第2期9-13,共5页Chinese Journal of Dialysis and Artificial Organs

基　　金：国家自然科学基金资助项目 ( 3 0 170 2 73)

摘　　要：本文研究了四种生物活性陶瓷人工骨替代材料 ,羟磷灰石 (HA) ,磷酸三钙 (TCP) ,胶原羟磷灰石 (CHA)及含氟羟磷灰石 (EHA)对血浆总蛋白 (TP)、纤维蛋白原 (FIB)及纤维粘连蛋白(FNC)细胞外基质的吸附情况 ,同时评价不同材料对血浆中钙、磷、镁的影响。结果发现四种材料吸附蛋白质的量有所不同 ,分别为 TCP>CHA>HA>FHA,三种蛋白质的变化规律基本一致 ,表明了吸附蛋白质的多少与材料的性能和蛋白种类有关。而血浆中钙、磷值有上升趋势 ,钙值分别为TCP>CHA>HA>FHA,而磷值分别为 HA>CHA>FHA>TCP,这可能与不同材料在生理环境中离子释放差异有关。We investigated the capabilities of four kinds of bioactive ceramics for artificial bone,hydroxyapatite(HA),β-tricalcium(TCP),collagen-hydroxyapatite(CHA) and fluoridated HA(FHA) to adsorb three kinds of plasma proteins,tolal protein (TP),fibrinogen(FIB) and fibronectin(FNC),and effect three elements,calcium(Ca),phosphorus(P) and magnesium(Mg) in plasma.The amount of protein adsorbed has relation to the character of protein and different materials.The capabilities of the biomaterials to adsorb plasma protein are TCP>CHA>HA>FHA.The effect of the materials to increase Ca in plasma is TCP>CHA>HA>FHA,and that to increase P is HA>CHA>FHA>TCP.We shall further study the effect of protein adsorption and element released on osteogenesis.

关 键 词：生物活性陶瓷材料 血浆蛋白吸附 钙 磷 影响 实验研究 

分 类 号：R318.08[医药卫生—生物医学工程]