RP-HPLC法测定复方双嘧达莫缓释片中双嘧达莫与阿司匹林的含量  

RP-HPLC Determination of Dipyridamole and Aspirin in Compound Dipyridamole Sustained-release Tablets

作　　者：杜云[1] 陆步实[1] 陆军[1] 

机构地区：[1]江苏省药物研究所,南京210009

出　　处：《江苏药学与临床研究》2003年第3期1-3,共3页Jiangsu Pharmacertical and Clinical Research

摘　　要：目的　建立高效液相色谱法测定复方双嘧达莫缓释片的含量测定方法。方法　用ODS C18柱 ;流动相以 0 0 2mol/LNa2 HPO4缓冲液 (2 0 %H3 PO4调节pH3 2 ) 乙腈 甲醇 (6 7∶2 8∶5 ) ;检测波长 2 2 7nm ;流量 1 0ml/min。结果　双嘧达莫的线性范围 :2 0 0 6～ 4 0 1 2 μg/ml(r=0 9995 ) ,平均回收率 10 0 8% ,RSD为 0 7% ;阿司匹林的线性范围 :2 892～ 5 7 84 μg/ml(r =0 9995 ) ,平均回收率 99 94 % ,RSD为 0 3%。结论　本法简便、灵敏、准确。Objective HPLC was used to determine dipyridamole and aspirin. Methods The chromatographic conditions are listed below:ODS column;0.02M dibasic sodium phosphate buffer(adjusted with phosphoric acid to a pH of 3.2)acetonitrile:methanol(67∶28∶5) as mobile phase,with detection at 227 nm and flow rate 1.0 ml/min. Results Dipyridamole: The linear range was 20.06-401.2 μg/ml( r =0.9995).The average recovery was 100.8%,RSD=0.7%;Aspirin: The linear range was 2.892～57.84 μg/ml( r =0.9995).The average recovery was 99.9%, RSD=0.3%. Conclusion This method is simple, sensitive and accurate.

关 键 词：RP—HPLC法 测定 复方双嘧达莫缓释片 双嘧达莫 阿司匹林 含量 

分 类 号：R927.2[医药卫生—药学] R971.1