农杆菌介导抗虫CpTI基因的花生遗传转化及转基因植株的再生  

Insect-resistant CpTI gene transferred into peanut (A.hypogaea L.) via Agrobacterium tumefaciens and regeneration of transgenic plantlets

作　　者：徐平丽[1] 单雷[1] 柳展基[1] 王芳[2] 张斌[1] 毕玉平[1] 张传坤[3] 

机构地区：[1]山东省农业科学院高新技术研究中心,山东济南250100 [2]山东师范大学生物系,山东济南250014 [3]山东省农业科学院蔬菜研究所,山东济南250100

出　　处：《中国油料作物学报》2003年第2期5-8,共4页Chinese Journal of Oil Crop Sciences

摘　　要：通过农杆菌(Agrobacteriumtumefaciens)Ti质粒介导途径,将抗虫CpTI基因转入花生,经过诱导分化获得转基因再生植株。PCR检测及Southern杂交鉴定结果表明,外源CpTI基因已整合到大部分再生花生植株的基因组中。通过抗虫性检测试验,转基因花生具有一定的抗虫性。Cowpea trypsin inhibitor (CpTI) gene was transferred into peanut (A.hypogaea L.) mediated by Ti plasmid of Agrobacterium tumefaciens LBA4404. Transgenic plants were obtained from transformed explants. Integration of CpTI gene into genome was confirmed through PCR and Southern blotting. The expression of the insect-resistance gene in the transgenic plants was observed through the bioassays.

关 键 词：花生 遗传转化 农杆菌介导 抗虫CpTI基因 转基因植株 植株再生 抗虫性 豇豆胰蛋白酶抑制剂基因 

分 类 号：S565.2[农业科学—作物学]