幽门螺杆菌hpaA基因RFLP研究  

RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP) OF ADHESIN GENE HPA A FROM DIFFERENT HELICOBACTER PYLORI STRAINS

作　　者：洪愉[1] 邹全明[1] 毛旭虎[1] 井申荣[1] 

机构地区：[1]第三军医大学医学检验系临床微生物和免疫学教研室,重庆400038

出　　处：《微生物学通报》2003年第3期42-45,共4页Microbiology China

基　　金：国家重点科技攻关计划项目(No 96 90 1 0 154);"863"生物与现代农业技术领域生物工程技术主题课题(No2001AA215161)

摘　　要：用限制性片段长度多态性 (RFLP)的方法评价幽门螺杆菌不同菌株鞭毛粘附素基因(hpaA)的变异性。PCR扩增 9株幽门螺杆菌 710bp的hpaA基因 ,用HhaⅠ、HaeⅢ限制性内切酶对该基因片段进行酶切分析。hpaA基因HaeIII单酶切可见 4种带型 ,HhaI单酶切出现 5种带型。从临床分离的H pylori菌株hpaARFLP互有差异 ,且不同于国际标准菌株 ;临床分离株感染动物后分离得到的动物适应株其hpaA基因也发生了变异。不同H pylori菌株间hpaA基因表现出明显的多态性 ,为开展HTo assess the variability of adhesin gene hpaA in differene H pyloristrains with PCR restriction fragment length polymorphism (RFLP) A 710 bp gene hpaA , obtained from 9 different H pylori strains, were digested by Hha Ⅰand Hae Ⅲ individually and analyzed by agarose gel electrophoresis Four different polymorphic types were found in hpaA digested with Hae III and five types with Hha I Clinical isolates of H pylori from Chongqing showed difference among them and remarkably distinguished from foreign standard strains Mongolia gerbil adapted H pylori strain,which were obtained from Mongolia gerbil infected with clinical isolate, also showed inconsistence in hpaA RFLP The hpaA gene from different H pylori strains revealed 1 variability, and this might provide an effective method for developing molecular epidemiology of H pylori

关 键 词：幽门螟杆菌 粘附素 HPAA RFLP 

分 类 号：Q933[生物学—微生物学]