溴氰菊酯对原代培养大鼠星形胶质细胞存活率和胞内游离钙浓度的影响  

Effects of deltamethrin on cell survival rate and intracellular free Ca^(2+) concentration in primary cultured astrocytes of rat

作　　者：吴又桐[1] 石年[1] 王素青[1] 李涛[1] 陈江海[1] 周丽[1] 陈亮[1] 董杰[1] 

机构地区：[1]华中科技大学同济医学院环境毒理学教研室

出　　处：《中华劳动卫生职业病杂志》2003年第3期194-196,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的　观察溴氰菊酯 (DM)对原代培养大鼠神经星形胶质细胞存活率及胞内游离钙离子浓度 ([Ca2 + ]i)的影响。方法　以锥虫蓝染料排斥试验检测细胞染DM后存活率的变化 ;以Fura 2 /AM为荧光指示剂 ,RF 5 30 1PC荧光分光光度计测定细胞内 [Ca2 + ]i的变化。结果　 1× 10 -5mol/L组染毒DM 72h后细胞存活率下降为 91.9% ,与对照组的差异有显著性 (P <0 .0 5 ) ,1× 10 -4mol/L组DM染毒4、12、4 8、72h后细胞存活率分别为 89.0 %、84 .8%、81.2 %和 79.2 % ,差异有显著性 (P <0 .0 1) ;1× 10 -7、1× 10 -6、1× 10 -5mol/L组DM染毒 5min后胞内 [Ca2 + ]i分别上升至 (45 1.4± 4 2 .3)、(5 36 .9± 4 7.5 )、(870 .9± 10 0 .5 )nmol/L ,与染毒前及对照组比 ,差异有显著性 (P <0 .0 1)。以后各染毒组胞内 [Ca2 + ]i逐渐下降 ,15min时 1× 10 -8、1× 10 -7、1× 10 -6、1× 10 -5mol/L染毒组胞内 [Ca2 + ]i分别降至 (12 4 .3± 6 .0 )、(131.3± 19.1)、(118.9± 1.4 )、(136 .6± 3.8)nmol/L ,与染毒前及对照组比差异均有显著性 (P <0 .0 5或P <0 .0 1)。结论　DM在体外可降低神经胶质细胞的存活率并短时升高胞内 [Ca2 + ]i。Objective To study the effects of deltamethrin(DM) on cell survival rate and intracellular Ca 2+ (i) concentration in primary cultured astrocytes of rat. Methods The cell survival rate was measured by Typan Blue assay;the intracellular i concentration was determined by the fluorescent Ca 2+ indicator Fura-2/AM. Results The survival rate of astrocytes was decreased to 91.9% after astrocytes were incubated with 1×10 -5 mol/L DM for 72 h( P <0.05).The cell survival rates were 89.0%,84.8%,81.2% and 79.2% respectively when astrocytes were administered with 1×10 -4 mol/L DM for 4,12,24 and 72 h,which were remarkably lower than control groups( P <0.01).Comparing with controls and before DM treatment,sharp increases in i concentration[(451.4±42.3),(536.9±47.5) and (870.9±100.5) nmol/L respectively] were observed when astrocytes were incubated with 1×10 -7 ,1×10 -6 and 1×10 -5 mol/L DM for 5 minutes( P <0.01).After astrocytes were treated with 1×10 -8 ,1×10 -7 ,1×10 -6 ,1×10 -5 mol/L DM for 15 minutes,the i concentrations were decreased to (124.3±6.0),(131.3±19.1),(118.9±1.4), (136.6 ±3.8) nmol/L respectively,which were significantly different from those of controls and before treatment.And this situation was almost keeping stable to 30 min. Conclusion The cell survival rate was decreased and the i concentration was temporarily increased when astrocytes were treated with DM.

关 键 词：溴氰菊酯 星形胶质细胞 钙离子 细胞存活率 

分 类 号：R114[医药卫生—卫生毒理学]