肝毛细线虫虫卵可溶性抗原诱导大鼠保护性免疫的研究  

PROTECTIVE IMMUNITY AGAINST CAPILLARIA HEPATICA INFECTION INDUCED BY SOLUBLE EGG ANTIGEN IN RATS

作　　者：黄慧聪[1] 金永国[1] 梁韶晖[1] 潘长旺[1] 秦茜[1] 邢文鸾[1] 易维平 

机构地区：[1]温州医学院寄生虫学教研室,浙江温州325003 [2]温州市疾病控制中心

出　　处：《中国寄生虫病防治杂志》2003年第3期149-151,共3页Chinese Journal of Parasitic Disease Control

摘　　要：目的　探讨肝毛细线虫虫卵可溶性抗原 (SEA)诱导保护性免疫的效果。　方法　 30只 SD大鼠随机分为 、 、 3组 ,每组 10只。 组和 组分别予以 0 .1ml4 0 0 μg/ 0 .1ml和 2 0 0 μg/ 0 .1ml SEA加等量的弗氏完全佐剂 (FCA)每周腹腔注射免疫大鼠 1次 ; 组为对照组 ,每周腹腔注射 0 .1ml生理盐水加等量的 FCA,共 3次。各组末次免疫 1周后 ,用约5 0 0粒 /鼠肝毛细线虫感染期虫卵感染大鼠 ,攻击感染 4周后 ,剖杀大鼠检获虫卵 ,计算每克肝卵 (L EPG)及减卵率 ,并检测大鼠血清特异性抗体 Ig G和血液嗜酸性粒细胞含量。　结果　 4 0 0 μg和 2 0 0 μg免疫原组减卵率分别是 5 0 .7%和 2 4 .9% ,两免疫组 L EPG、抗体水平及血液嗜酸性粒细胞含量均高于对照组 (P<0 .0 1)。　结论　肝毛细线虫 SEA可诱导大鼠产生保护性免疫。Objective To explore protective immunity effect against Capillaria hepatica infection induced by soluble egg antigen (SEA) in rats. Methods 30 SD rats were randomly divided into Ⅰ, Ⅱ and Ⅲ groups. In group Ⅰ, each rat was immunized with 0.1 ml 400 μg/0.1 ml SEA mixed with 0.1 ml FCA; In group Ⅱ, each rat was immunized with 0.1 ml 200 μg/0.1 ml SEA mixed with 0.1 ml FCA; In group Ⅲ, each rat was immunized with 0.1 ml physiological saline mixed with 0.1 ml FCA as control. All rats in the three groups were immunized by intraperitoneal injection once a week for 3 times. A week after the final injection, all rats were challenged with 500/mouse infective stage egg. 4 weeks later, rats were killed and the LEPG were obtained and counted. The serum antibody and the eosinophils of experimental rats were carried out as well. Results In group Ⅰ and Ⅱ, the egg reduction rates were 50.7% and 24.9% respectively. The LEPG, the antibody A levels and the eosinophils were increased in the two immunized groups compared with those in control group respectively(P<0.01). Conclusion SEA of C. hepatica could induce protective against the infection of C. hepatica in rats.

关 键 词：肝毛细线虫 虫卵 可溶性抗原 大鼠 保护性免疫 生理盐水 肝毛细线虫病 

分 类 号：R383.1[医药卫生—医学寄生虫学] R532.1[医药卫生—基础医学]