二仙汤加减对实验性脑缺血大鼠心房特殊颗粒影响的体视学研究  

Morphometric Study on Influence of Modified “Two Immortals Decoction" on Atrial Specific Granules in Rats with Experimental Cerebral Ischemia

作　　者：王洪海[1] 高慧英[1] 崔海庆[1] 李亚鲁[1] 李善刚[1] 

机构地区：[1]泰山医学院,山东泰安271000

出　　处：《上海中医药大学学报》2003年第2期47-50,共4页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：山东省教育委员会资助项目 (J96K2 1)

摘　　要：探讨二仙汤加减对实验性脑缺血大鼠心房特殊颗粒 (ASG)的影响及其意义。中老年Wistar大鼠 ,采用双侧颈总动脉夹闭 2h后去夹再通的方法 ,制作急性不完全性脑缺血再灌模型。实验组大鼠造模前给予二仙汤加减煎剂灌胃3d ,于血流再灌后 6h、2 4h、3d、7d处死。取大鼠右心耳制作超薄切片 ,电镜观察摄片并进行体视学测试。结果 :再灌6h、2 4h组对照组大鼠右心耳ASG明显减少 ,实验组仅少量释放。体视学测试统计结果显示 :ASG的VV、NA、NV 及D实验组与对照组比较有显著差异。结论In order to explore influence and significance of modified “Two Immortals Decoction' on atrial specific granules in the rats with experimental cerebral ischemia, acute incomplete cerebral ischemia models were established by clipping bilateral common carotid arteries for two hours in the middle and old-aged Wistar rats. Before modeling, the experimental rats were given liquid medicine of this formula and were killed at 6 th hour, 24 th hour, 3 rd day and 7 th day respectively after perfusion of blood flow, to take out the right atrial appendages for ultra-thin sections for morphometric determination by electron microscope. The results showed that ASG obviously decreased in the group with 6h and 24h reperfusion than in the control group, and there was only a little release in the experimental group. The results of the morphometric determination and statistics showed that there was a significant difference between the experimental group and control group in Vv, N A, Nv and ? of ASG. Conclusion: “Two Immortals Decoction” can remarkably decrease the release and consumption of ASG in the myocardial fibers of the right atrial appendages in the rats with acute incomplete cerebral ischemia.

关 键 词：二仙汤加减 实验 脑缺血 大鼠 心房特殊颗粒 影响 再灌损伤 脑保护 

分 类 号：R285.5[医药卫生—中药学]