广东省传染性非典型肺炎病原体的分离鉴定及检测结果分析  

Etiological analysis of SARS cases in Guangdong province

作　　者：黄吉城[1] 林锦炎[1] 万卓越[1] 郑焕英[1] 陈秋霞[1] 张欣[1] 李晖[1] 郑夔[1] 鄢心革[1] 方苓[1] 周惠琼[1] 江立敏[1] 钟豪杰[1] 黄平[1] 陈经雕[1] 刁丽梅[1] 张万里[1] 谢韶英[1] 柯昌文[1] 张勤奋[2] 崔金明[2] 黄小俊[2] 张景强[2] 

机构地区：[1]广东省疾病预防控制中心微生物检验所,广州510300 [2]中山大学生物防治国家重点实验室

出　　处：《华南预防医学》2003年第3期29-31,T001,共4页South China Journal of Preventive Medicine

摘　　要：目的　对引起广东省传染性非典型肺炎 (SARS)的病原体进行分离鉴定 ,并分析各市首发病例、死亡病例、各个阶段病例的检测情况 ,为该病的诊断和防治提供依据。方法　对 2 0 0 3年 1～ 5月份采自广州、肇庆、江门、汕头、中山、河源、珠海等地的SARS病例的咽拭子、咽漱液等标本 ,用Vero E6、Vero、MDCK、Hep 2、传代人胚肺 5种细胞进行分离培养 ,并使用电镜、间接免疫荧光 (IFA)、PCR及基因序列测定等方法对分离物进行鉴定 ,应用ELISA、荧光定量PCR等技术分别对各个阶段SARS病例的血清及咽拭子等标本进行检测。结果　 5种细胞接种上述病人咽拭子标本后 ,均有部分出现细胞病变 ,电镜观察可找到冠状病毒样颗粒。分离物扩增出冠状病毒特异基因片断。分离物与病人恢复期血清抗体发生特异性反应。 1～ 5月的病例标本SARS冠状病毒PCR阳性率分别为 1 0 0 0 0 % (2 / 2 )、76 1 9%(1 6 / 2 1 )、71 74 % (33/ 4 6 )、2 0 0 0 % (1 8/ 90 )、6 36 % (1 1 / 1 73) ;SARS冠状病毒抗体IgG阳性率分别为 1 0 0 0 0 % (2 / 2 )、78 5 7% (1 1 / 1 4 )、5 8 33% (1 4 / 2 4 )、30 3% (1 0 / 33)、1 2 5 0 % (9/ 72 )。河源、佛山、肇庆、中山、东莞、珠海六市首发病例均检测出SARS冠状病毒IgG抗体。 1Objective To identify the pathogen of SARS in Guangdong province. Methods The throat swabs of SARS cases were incubated on several cells including Vero E6, Vero, MDCK, Hep 2, HL to isolate the pathogen. And the pathogens were identified through electronic microscope, IFA, PCR and DNA sequencing. Then, the throat swab and serum samples collected from SARS case were tested for SARS coronavirus by PCR and ELISA respectively. Results\ The hypothesis that a novel coronavirus was the main pathogen of SARS was supported by DNA sequence, electronic microscope, and serum test. The positive rate of PCR was 100%(2/2)、76.19%(16/21)、71.74%(33/46)、20.00%(18/90)、6.36%(11/173) and the positive rate of the SARS CoV IgG was 100%(2/2)、78.57%(11/14)、58.33%(14/24)、30.3%(10/33)、and 12.50%(9/72) from the samples collected from January to May, respectively. Conclusion\ SARS corona virus is the main pathogen of the epidemic in Guangdong province; The positive rates of specific gene and IgG were different with the patients at the different epidemic periods.

关 键 词：广东 传染性非典型肺炎 病原体 分离 鉴定 检测 严重急性呼吸综合征 

分 类 号：R563.1[医药卫生—呼吸系统]