禽副粘病毒2型Yucaipa标准株F基因的克隆和序列分析  

Molecular cloning and sequence analysis of the fusion glycoprotein of avian paramyxovirus type 2 viruses-strain Yucaipa

作　　者：卜春亚[1] 赵继勋[1] 杨爱梅[1] 

机构地区：[1]中国农业大学实验动物研究所,北京100094

出　　处：《中国预防兽医学报》2003年第4期254-257,共4页Chinese Journal of Preventive Veterinary Medicine

摘　　要：根据从GenBank上查到的PMV_2型Yucaipa株F基因序列———D1 3 977序列 ,设计特异性引物 ,从禽副粘病毒 2型Yucaipa标准株成功地克隆了F基因全编码序列。所测的核苷酸序列大小为 1 65 4bp,编码一个含 5 3 6个氨基酸残基的多肽 (未加工前 ) ,分子量约为 5 5 .75kDa。裂解位点在F基因的第 98和第 99个氨基酸残基之间 ,F2蛋白羧基端裂解位点区的氨基酸残基序列为Lys_Pro_Ala_Ser_Arg。F基因序列同源性比较结果表明本研究所测的序列是PMV_2型F基因序列 ,并进一步证实了副粘病毒 2型与分离自猴的Murayama病毒在系统进化上有非常紧密的关系。According to GenBank D13977 sequence, F gene of PMV_2 strain Yucaipa was successfully cloned and sequenced. The sequenced F gene is 1 654 nucleotide long and contain a single open reading frame encoding a polypeptide of 536 amino acids with a predicted molecular weight (MW) of 55.75 kDa. F0 protein is cut into F2 and F1 protein between the 98th and 99th amino acid residue. The F2/F1 cleavage site in carboxyl terminus of F2 protein is Lys_Pro_ Ala_ Ser_ Arg, which is similar to that of Murayama virus (Lys_Pro_Thr _ Ala _Arg). Comparative analysis of F gene sequences of PMV_2 viruses showed that the sequence was PMV_2 F gene sequence. Murayama virus isolated from Cynomologus Monkeys was also proved to have close relationship with paramyxovirus type 2 by comparing F genes of PMV_2 with that of Murayama virus.

关 键 词：副粘病毒2型 F基因 RT-PER 序列分析 

分 类 号：S852.65[农业科学—基础兽医学] Q78[农业科学—兽医学]