气相色谱法测定健康人含服速效救心丸后冰片的血药浓度  

Determination of Borneol in Blood After Keeping Suxiao Jiuxin Pills in Healthy People's Mouth by GC

作　　者：吴红[1] 郭军[2] 李晓晔[1] 黄熙[2] 

机构地区：[1]第四军医大学基础部化学教研室,西安市710032 [2]第四军医大学西京医院,西安市710032

出　　处：《中国药房》2003年第7期414-415,共2页China Pharmacy

基　　金：国家自然科学基金项目(39870932)

摘　　要：目的 :用气相色谱法测定健康人含服速效救心丸后冰片 (龙脑、异龙脑)的血药浓度。方法 :采用HP -5石英弹性毛细管柱 ,柱温为85℃ ,汽化室温度为180℃ ,检测器温度为200℃ ,载气为氮气 ,萘为内标。结果 :冰片进样量在50～1000ng/ml范围内其峰面积与进样量呈良好线性关系 ,Y=—0 1273+0 0015X ,回收率为 (98 7±2 31) % ,RSD=2 76% (n=6)。健康人血清中冰片含量在15min左右达血药浓度高峰。结论 :建立的气相色谱法可靠、灵敏、简便 。OBJECTIVE:To determine the concentration of borneol in blood after keeping Suxiao Jiuxin pills in healthy men's mouth by GC METHODS:HP-5 quartz elasticity capillary column(15m×0 53mmID) was used with nitrogen as the carrier gas,temperature of column was 85℃ The injector temperature and detector temperature were 180℃,200℃ respectively Naphthalene was used as internal standard RESULTS:The standard curve was linear within the concentration range of 50～1 000ng/ml of borneol,the calibrating equation was Y=-0 1 273+0 0 015X The recovery was (98 7±2 31)%,(RSD=2 76%,n=6) The peak of blood drug concentration was obtained in 15 min or so CONCLUSION:The method was simple,rapid,accurate and suitable for pharmacokinetic study of preparation containing borneol

关 键 词：气相色谱法 测定 健康人 含服 速效救心丸 冰片 血药浓度 龙脑 异龙脑 

分 类 号：R972[医药卫生—药品]