高效液相色谱法测定复方黄连颗粒中葛根素的含量  

Determination of the Content of Puerarin in Compound Rhizoma Coptidis Graininess by HPLC

作　　者：唐丽琴[1] 刘圣[1] 陈礼明[1] 李矗[1] 

机构地区：[1]安徽省立医院药剂科,合肥市230001

出　　处：《中国药房》2003年第7期425-427,共3页China Pharmacy

基　　金：安徽省重点科研项目 (01023018)

摘　　要：目的 :建立复方黄连颗粒中葛根素含量的测定方法。方法 :采用高效液相色谱法 ,色谱柱为C18(4 6mm×250mm ,5μm) ,检测波长为250nm ,流动相为甲醇 -水 (30∶70) ,流速为1 0ml/min ,柱温为30℃。结果 :葛根素在0 312μg～1 560μg范围内线性关系良好 (r=0 9989)。葛根素的平均回收率为99 2 % (RSD=2 08% ,n=5)。结论 :本方法具有灵敏度高 ,操作简单、稳定 ,分离效果好等优点 。OBJECTIVE:To establish a method for determination of the content of puerarin in compound Rhizoma Coptidis graininess METHODS:HPLC method was used The samples were separated by C18 (4 6mm×250mm,5μm) column with the mobile phase of methanol-water(30∶70) The detection wavelength was set at 250nm Flow rate was 1 0ml/min Column temperature was 30℃ RESULTS:The linear ranges of puerarin was 0 312μg～1 560μg The average recovery was 99 2%,RSD=2 08%(n=5) CONCLUSION:The method is sensitive,rapid and accurate and suitable for the determination of puerarin in compound Rhizoma Coptidis graininess

关 键 词：高效液相色谱法 复方黄连颗粒 葛根素 含量测定 中药 

分 类 号：R286.0[医药卫生—中药学]