骨折端Ⅷ因子相关抗原与血流量的实验研究  

An experimental study of the correlation of blood coagulation factor Ⅷ-related antigen and blood flow at the fracture ends

作　　者：初同伟[1] 王正国[2] 朱佩芳[2] 焦文仓[1] 温建良[3] 

机构地区：[1]第三军医大学新桥医院骨科,重庆400042 [2]大坪医院野战外科研究所 [3]大坪医院

出　　处：《中国骨伤》2003年第1期17-19,共3页China Journal of Orthopaedics and Traumatology

基　　金：国家重点基础研究发展规划资助项目 (G1 9990 542 0 4 )

摘　　要：目的　研究骨折愈合过程中骨折端Ⅷ因子相关抗原和血流量变化的规律及其相关性。方法　选用 35只大耳白兔制作左侧桡骨骨折动物模型 ,分别于伤后 8、2 4、72h ,1、3、5、8周利用单光子放射计算机断层显像术 (SinglePhotonEmissionComputerizedTomography ,SPECT)测定骨折端血流量变化 ,之后取骨折端标本提取蛋白样本定量检测Ⅷ因子相关抗原。结果　骨折端血流量在致伤后 8h开始上升 ,1周时达顶峰 ,高血流量状态一直维持到第 3周 ,第 5和第 8周基本恢复到正常水平 ;骨折端Ⅷ因子相关抗原在伤后 2 4h开始增高 ,骨折后 3天达到较高的水平 ,该状态一直维持到骨折后的第 3周 ,到第 5周时开始出现较明显的回落 ,第 8周时基本恢复到正常水平。结论　伤后 72h骨折端血流量增高与骨折端血管生成有关 ,早期的血流量增加可能与骨折后的创伤反应有关。Objective To study the changing pattern and correlation of blood coagulation factor Ⅷ-related antigen and blood flow at the fracture ends in the process of fracture healing Methods Thirty five rabbits were selected to establish the fracture model at the left radius;the blood flow was estimated by single photon emission computerized tomography (SPECT) at different intervals (8th,24th,72th hour,1st,3rd,5th,8th week)after fracture,then the specimens were taken to procure protein for assay the changing blood coagulation factor Ⅷ-related antigen.Results The blood flow began to increase at the 8th hour after fracture and reached the peak on the lst week;the condition of high blood flow kept to the third week and dropped to normal level at the 5th and 8th week after fracture.Factor Ⅷ-related antigen began to increase at the 24th hour after fracture and reached to a higher level at the 72th hour;this state was kept to the 3rd week.The factor Ⅷ-related antigen obviously decreased at the 5th week after fracture and dropped to the normal at the 8th week.Conclusion The increase of blood flow at fractured sits is related to the angiogenesis after fracture in 72 hours,the increasing of blood flow within 3 days may be related to the traumatic reaction.

关 键 词：Ⅷ因子相关抗原 血流量 实验 相关性 桡骨骨折 动物模型 

分 类 号：R683.41[医药卫生—骨科学]