纳米银胶与蛋白质的相互作用及应用  

INTERACTION OF NANO-SILVER COLLOID WITH BOVINE SERUM ALBUMIN AND ITS APPLICATION

作　　者：夏传俊[1] 张莉[1] 张锐[1] 赵广超[1] 

机构地区：[1]安徽师范大学环境科学学院,安徽芜湖241000

出　　处：《安徽师范大学学报（自然科学版）》2003年第2期146-148,共3页Journal of Anhui Normal University(Natural Science)

基　　金：安徽省自然科学基金资助项目(01044702)

摘　　要：在水溶液中,纳米银粒子的紫外吸收峰随牛血清蛋白(BSA)浓度的增加而红移并且吸光度降低,表明纳米银粒子能与BSA发生相互作用,红外光谱也证实这种作用的存在.水溶液中,纳米银粒子具有一个较弱的反二级散射光谱,BSA存在下,其反二级散射大大增强并产生相应的散射光谱,最大波长位置位于470nm.BSA在0-8μg/ml范围内与散射峰强度△I成正比.方法有很高的灵敏度,可用于痕量BSA的测定.In aqueous, the UV-Vis spectrum of nano-silver shifted to red wavelength and the absorbance decreased with increasing the concentrations of bovine serum albumin (BSA). This revealed that nanosilver was able to interact with BSA, which was testified by IR spectrum. In aqueous, the antisecondorder scattering (ASOS) of nanosilver was very faint. However, in the presence of BSA, the scattering was enhanced greatly. The maximum ASOS was at 470 nm and the intensities of ASOS were directly proportional to the concentrations of BSA in the range of 08.0μg/mL. The further investigation can develop a new method with high sensitivity for the determination of trace amounts of BSA.

关 键 词：纳米银胶 纳米银粒子 牛血清蛋白 相互作用 反二级散射光谱 共振散射光谱 

分 类 号：Q512.103[生物学—生物化学] TB383[一般工业技术—材料科学与工程]