中华鳖卵泡免疫组织化学研究  

IMMUNOHISTOCHEMICAL STUDIES ON THE FOLLICLE OF PEOLDIESCUS SINENSIS

作　　者：蔡亚非[1] 傅兆水 王海涛[1] 刘再群[1] 郝家胜[1] 陈发扬[1] 

机构地区：[1]安徽师范大学生命科学学院,安徽芜湖24100 [2]南京市红山森林动物园,江苏南京210028

出　　处：《安徽师范大学学报（自然科学版）》2003年第2期155-158,共4页Journal of Anhui Normal University(Natural Science)

基　　金：安徽省高校优秀青年教师基金资助(No.2000jpw107)

摘　　要：应用六种特异性抗血清(抗EGF、NGF、TGFβ1、HCG、AFP、P53)对中华鳖卵巢内卵泡细胞进行免疫组织化学定位.结果发现:NGF免疫反应出现在颗粒细胞及卵泡鞘.AFP、EGF、HCG阳性细胞均在胚床中发现,TGFβ1在卵泡鞘中被发现,P53阳性细胞在早期卵泡细胞中发现.由此探讨各种因子与卵泡发育的功能关系以及各因子见的相互作用及调控蛋白的时序性表达对其影响.Immunochemical method was applied to locate correspodent substances in the follicle of Peoldiscus sinensis with six antisera (antiEGF, NGF, TGFβ1, HCG, AFP ,P53).Results showed that NGF immunoreactivity emerged in the granular cells and the thecal layer and AFP, EGF , HCG positive cells were all found in the germinal bed, TGFβ1 positive cells were found in thecal layer, and P53 positive cell came into being in the early follicular cells .Thus the correlation between the growth factors and the folliculogeneis ,the mutual influence among the growth factors ,the sense of regulative proteins' expression at particular time were discussed.

关 键 词：中华鳖 卵巢 卵泡细胞 免疫组织化学 颗粒细胞 卵泡鞘 胚床 卵泡发育 爬行动物 

分 类 号：Q959.63[生物学—动物学] Q954.43