LaCl_3对大豆Cu,Zn-SOD活性影响研究  

Influence of LaCl_3 on Activity of Cu, Zn-SOD in Soybean

作　　者：潘登奎[1] 张金桐[1] 杨家朴[1] 

机构地区：[1]山西农业大学文理学院,山西太谷030801

出　　处：《中国稀土学报》2003年第3期339-342,共4页Journal of the Chinese Society of Rare Earths

基　　金：山西省自然基金资助项目

摘　　要：研究证明0～100μmol·L-1的LaCl3对于大豆Cu,Zn SOD的NBT光还原活性抑制作用具有明显影响,即受La3+作用,大豆Cu,Zn SOD活性动力学曲线为S形,同时受La3+作用Cu,Zn SOD的热稳定性明显提高。大豆Cu,Zn SOD受La3+作用的阈值浓度和饱和浓度分别为cLa3+=18μmol·L-1,cLa3+=65μmol·L-1。根据Hill系数3.9和荧光光谱分析,大豆Cu,Zn SOD分子中存在4个La3+的强结合位点。证明了La3+对Cu,Zn SOD具有明显的正协同效应。In recent years, the rare earths(RE) elements have been applied to medical science. The RE playes some positive actions in cancerresistance, antiinflammation, antiinbacterial, anticoagulation of blood, treating burn, taking part in immune process and so on.The effects of RE compounds on enzymes and lipid peroxidation process were reported.The superoxide dismutase(SOD) plays a very important role in the metabolism of bioactive oxygen, the superoxide theory regarding to the bioposion mechanism was established. The activity and thermostability of Cu, ZnSOD under LaCl3 action was studied. The results indicate that LaCl3 treatment with a level of 0～100 μmol·L-1 has a noticeable effect on the NBT photoreduction inhibitory action of Cu, ZnSOD in soybean. The active kinetic curve of Cu, ZnSOD shapes 'S' and thermostability rises apparently under the action of La3+. The threshold concentration and saturation concentration of Cu, ZnSOD subjected to the action of La3+ are 18 and 65 μmol·L-1 respectively. The conclusion is that there are 4 points to combine La3+ in Cu, ZnSOD reaches on the basis of analyses of Hill coefficent(3.9) and fluorescence spectrum. The study proved that the La3+ has an obvious positive syneryism on Cu, ZnSOD.

关 键 词：植物学 氯化镧 Cu Zn—SOD Hill系数 正协同效应 稀土 

分 类 号：O614.33[理学—无机化学]