柑桔种间体细胞杂种的花粉特性及贮藏研究  

Study on the Pollen Characteristics and Storage of Citrus Interspecific Somatic Hybrid

作　　者：刘艳玲[1] 夏仁学[1] 李国怀[1] 马梦亭[1] 伊华林[1] 

机构地区：[1]华中农业大学园艺林学学院

出　　处：《武汉植物学研究》2003年第3期254-258,共5页Journal of Wuhan Botanical Research

基　　金：国务院三峡建设委员会移民科技项目;国家 948项目资助。

摘　　要：对粗柠檬与“哈姆林”甜橙种间体细胞杂种的花粉特性 (形态、花粉量、生活力等 )进行了观察、检测 ,并于- 2 0℃的低温条件下开展了贮藏试验。结果表明 :花粉离体萌发的适宜培养基为 0 .8%琼脂 +16 %蔗糖 +10 m g/kg硼酸 ,萌发率为 15 .31% ,其生活力介于两亲本之间 ,可作为杂交亲本进一步被利用。贮藏试验结果显示 :以真空、干燥 (Ca Cl2 或硅胶作干燥剂 )贮藏花粉的效果较好。授粉试验证明 ,贮藏花粉与新鲜花粉的座果率差异不显著 ,说明低温、真空和干燥是贮藏粗柠檬与“哈姆林”甜橙种间体细胞杂种花粉的较适条件。The pollen characteristics of Citrus interspecific somatic hybrid 'Hamlin sweet orange (Citrus sinensis) +Rough lemon (Citrus jambhiri)' was observed. The results showed that the medium containing 16% sucrose, 10 mg/kg H 3BO 4 and 0 8% agar in vitro culture was suitable for pollen germination, and the pollen germinability of 'Hamlin sweet orange+ Rough lemon' was 15 31%, which indicated that the viability of interspecific somatic hybrid was intermediate to its parents and could be used as parent in future crossing. Pollen grains of 'Hamlin sweet orange+Rough lemon' were stored at -20℃. The results showed that vocauum and dry condition (calcium chloride or silica gel as desiccants ) were benefit for the pollen storage. And the results of artificial pollination also showed that the fruit set with fresh pollen and stored pollen was not significantly different. Thus low temperature, vocauum and dry condition were benefit for the pollen storage of Citrus somatic hybrid.

关 键 词：柑桔 种间体细胞杂种 花粉特性 花粉贮藏 

分 类 号：Q945[生物学—植物学] S666[农业科学—果树学]