库尔勒香梨上ACLSV的RT—PCR检测  

RT-PCR Detection of ACLSV in Korla's Xiangli Pear Variety

作　　者：牛建新[1] 刘连科[1] 朱军[1] 覃伟铭[1] 吴忠华[1] 王小兵[1] 

机构地区：[1]新疆石河子大学农学院园艺系,新疆石河子832003

出　　处：《果树学报》2003年第4期243-246,共4页Journal of Fruit Science

基　　金：国家自然科学基金(30060053);教育部科学技术研究重点项目(02180);兵团科委项目(NKB02SDXNK01SW)

摘　　要：以库尔勒香梨叶片和皮层为材料通过改进提取方法提取总RNA,获得了较完整的RNA,在此基础上进行了反转录和PCR扩增。并进行了库尔勒香梨上ACLSV(Apple chlorotic leaf sopt virus)的RT-PCR检测,建立了该病毒的RT-PCR检测体系。用此体系扩增得到了ACLSV一个长约为358bp的片段。In order to obtain high purity and integrality of RNA from the fresh leaf and bark of Korla's Xiangli pear variety infected with ACLSV(Apple chlorotic leqf sopt virus) , the extraction was improved by added 1% CTAH, 2% PVP, 2% 2-mercaptoethanol (added just before use) to the extraction buffer and lowed the concentration of ethanol to deposit amylose. Using total RNA as template, reverse transcriptase-polymerase reaction (RT-PCR) was performed, and RT-PCR detection technology of ACLSV in Korla's Xiangli pear variety was reported in this paper. PCR amplification conditions were optimized , and the optimum RT-PCR system of ACLSV was developed. A 358 bp nucleotide fragment of ACLSV was obtained by using the optimum system.

关 键 词：库尔勒香梨 ACLSV RT-PCR 

分 类 号：S661.2[农业科学—果树学]