二花脸和大白猪的脂肪组织中GH-R、IGF-1和IGF-ⅠR基因表达的发育性变化  

The Developmental Patterns of GH-R,IGF-1 and IGF-ⅠR Gene Expression in Adipose Tissue of Erhualian and Large White Pigs

作　　者：周杰[1] 赵茹茜[1] 韦习会[1] 夏东[1] 胥清富[1] 陈杰[1] 

机构地区：[1]南京农业大学农业部动物生理生化重点开放实验室

出　　处：《Acta Genetica Sinica》2003年第7期657-662,共6页

基　　金：国家自然科学基金重点项目资助~~

摘　　要：分别于出生当天、3、2 0、30、4 5、90、12 0、180日龄随机屠宰二花脸公、母猪各 4头 (30日龄仅有公猪 ) ,于 2 0、30、90、12 0、180日龄随机屠宰大白猪公猪 4头 ,采集背部皮下脂肪组织。用RT PCR方法 ,以 18SrRNA作内标 ,定量分析脂肪组织中生长激素受体 (GH R)、胰岛素样生长因子 1(IGF 1)及胰岛素样生长因子Ⅰ型受体 (IGF ⅠR)基因表达的发育性变化 ,并进行品种和性别间比较。结果表明 :脂肪组织中GH RmRNA的表达有明显的发育性变化及品种差异 ,二花脸母猪GH RmRNA水平出生时较低 ,4 5日龄达到高峰 ,之后维持稳定 ;二花脸公猪GH RmRNA水平在出生时较高 ,至 4 5日龄达到最高值 ,之后显著下降 ,但总体上没有性别差异 ;大白猪公猪GH RmRNA水平极显著低于二花脸公猪 (P <0 0 1)。脂肪组织中IGF 1mRNA的表达有明显的发育性变化及性别和品种差异 ,二花脸母猪显著低于公猪 (P <0 0 5 ) ,大白猪公猪极显著低于二花脸公猪 (P <0 0 1) ;脂肪组织中IGF ⅠRmRNA的表达有明显的发育性变化 ,但在总体上没有明显的性别和品种差异。结果提示 :猪脂肪组织中GH R、IGF 1和IGF ⅠR的基因表达有特定的发育模式 ,IGF 1基因表达的品种差异可能正是两品种猪脂肪沉积规律不同的主要原因之一。Growth hormone (GH) is essential for postnatal growth in animal,it regulates numerous cellular functions by direct effect on its receptor (GH R) in many different tissues.In present experiment single tube semi quantitative RT PCR was applied to investigate the developmental patterns of GH R,IGF 1 and IGF ⅠR mRNA expression in dorsal subcutaneous adipose tissue of Erhualian and Large White pigs,and 18S internal standards were used as control.Eight Erhualian pigs were sampled at birth,3,20,30,45,90,120,180 days of age respectively,four Large White boars were sampled at 20,30,90,120,180 days of age respectively.The results showed that GH R mRNA levels in adipose tissue were changed with age ( P <0 05).The expression of GH R mRNA in Erhualian gilts was low at birth but reached a peak at 45 days of age,and kept constant thereafter.The expression of GH R mRNA in Erhualian boars was high at birth and reached a peak at 45 day,then decreased remarkably.It had no differences between sexes in general.The expression of GH R mRNA in Large White boars was lower than that in Erhualian boars ( P <0 01).The IGF 1 mRNA expression in adipose tissue was changed with age.The IGF 1 mRNA expression in Erhualian gilts was lower than that in Erhualian boars ( P <0 05),and the IGF 1 mRNA expression in Large White boars was lower than that in Erhualian boars ( P <0 01).The IGF ⅠR mRNA expression in adipose tissue was changed with aging,but it had no differences between sexes and breeds in general.The results suggest that the GH R,IGF 1 and IGF ⅠR mRNA expression in adipose tissue follow specificdevelopmental pattern,and the different patterns of IGF 1 gene expression may contribute to different sediment of adipose tissue between two breeds.

关 键 词：猪 脂肪组织 生长激素受体 胰岛素样生长因子-1 胰岛素样生长因子I型受体 

分 类 号：S828[农业科学—畜牧学]