一种新的蝎毒素BmTXKβ的基因组结构及分析  

Analysis of the Genomic Organization of A Novel Scorpion Toxin BmTXKβ

作　　者：肖帆[1] 曹志贱[1] 吕猛[1] 蒋达和[1] 毛歆[1] 刘辉[1] 李文鑫[1] 

机构地区：[1]武汉大学生命科学学院,武汉430072

出　　处：《Acta Genetica Sinica》2003年第7期663-667,共5页

基　　金：国家自然科学基金 (批准号 :3 9970 897)资助项目~~

摘　　要：根据从东亚钳蝎 (ButhusmartensiiKarsch ,BmK)毒腺cDNA文库中筛选得到的长链钾离子通道毒素BmTXKβ基因序列设计引物 ,采用Vectorette方法克隆其 5′及 3′侧翼区。结果表明 :BmTXKβ在编码信号肽的基因序列中有一大于 997bp的内含子 ,并且其成熟肽中也有一个 886bp内含子 ,而目前已知的其他蝎毒素只在信号肽中有一个内含子或没有内含子。Scorpion venoms contain different types of low molecular mass toxic peptides acting on ion channels.Many cDNAs and genomic genes encoding these toxins have been isolated and sequenced while the mechanism of expression and regulation of scorpion toxins is not well studied yet.BmTXKβ,one of the four putative long chain potassium channel toxins,is isolated from the cDNA library of the venom gland of Chinese scorpion BmK ( Buthus martensii Karsch).It has an 886 bp intron located in the mature peptide while other scorpion toxins' introns are located in the signal peptide.The special genomic organization of BmTXKβ makes it a good object to study the mechanism of expression and regulation of scorpion toxins.With primers designed according to the already known sequence of BmTXKβ,its 5' and 3' flanking regions are cloned by the Vecttorette II Staorette Pack method and sequenced.Analysis of the sequence shows that another intron longer than 997 bp is located in the signal peptide region of BmTXKβ,which makes BmTXKβ different from all the other scorpion toxins that have no intron or only one intron in the signal peptide.The special genomic organization of BmTXKβ indicates that BmTXKβ is a new membership of long chain potassium channel toxin.

关 键 词：东亚钳蝎 K^+通道毒素 BmTXKβ 基因组织结构 

分 类 号：Q953[生物学—动物学]