rhBMP-2及rhbFGF对骨髓间充质干细胞增殖的长期效应的实验研究  

The Long-term Effects of rhBMP-2 and rhbFGF on Proliferation of Bone Mesenchymal Stem Cells.

作　　者：侯锐[1] 毛天球[1] 陈书军[1] 陶凯[1] 陈富林[1] 赵丽君[1] 

机构地区：[1]第四军医大学口腔医学院颌面外科,陕西西安710032

出　　处：《口腔医学研究》2003年第3期166-169,共4页Journal of Oral Science Research

基　　金：国家"973"基金项目资助;编号 :G19990 5 430 8

摘　　要：目的 :检测rhBMP - 2和rhbFGF单独、联合及序贯作用对兔骨髓间充质干细胞增殖的长期效应。方法 :在细胞培养技术下 ,采用MTT法检测单独、联合及序贯施加rhBMP - 2和rhbFGF后 ,兔骨髓间充质干细胞在第 1,3 ,5 ,7及 10天增殖的状况 ,并进行统计学分析。结果 :rhBMP - 2和rhbFGF均能长期显著地促进兔骨髓间充质干细胞的增殖 ,并呈剂量依赖性。两种生长因子联合作用的效应高于单独或序贯作用的效应。结论Objective:To evaluate the long-term effects of rhBMP-2 and rhbFGF on proliferation of rabbit bone mesenchymal stem cells by using them alone, associatedly and sequentially.Methods:Cell culture technique and MTT colorimetric assay were used to evaluate the proliferation of rabbit bone mesenchymal stem cells on the 1st, 3rd, 5th, 7th, and 10th day by adding rhBMP-2 and rhbFGF on different ways.Results:Statistical analysis showed that both rhBMP-2 and rhbFGF could enhance the proliferation of rabbit bone mesenchymal stem cells significantly in long term, and also in a dose-dependent manner.The effects of the two growth factors were significantly higher by using them associatedly than by using them alone or sequentially.Conclusions:In tissue engineering work, we can get a great quantity seed cells in a short time by using different growth factors associatedly.

关 键 词：骨髓间充质干细胞 细胞增殖 重组骨形成蛋白-2 碱性成纤维细胞生长因子 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]