Secondary Structure and Neurotrophic Effect of a 33.1 kDa Specific Protein (SSP-33.1) in Spinal Sensory Ganglia  

感觉神经33.1kDa特异蛋白二级结构及其神经营养活性探讨(英文)

作　　者：沈建英[1] 俞庆声[1] 王琪[1] 李泉[1] 蒲小平[1] 

机构地区：[1]北京大学药学院分子与细胞药理学系,100083

出　　处：《Journal of Chinese Pharmaceutical Sciences》2003年第2期106-111,共6页中国药学（英文版）

基　　金：SupportedbytheNationalProgramforPreperiodKeyBasicResearchProjects

摘　　要：Aim To analyze the secondary structure and neurotrophic effect of a specific protein in sensory neurons. Methods Comparison of the proteins expressed in the rat spinal sensory neurons and motor neurons was made by two dimensional electrophoresis. One specific protein in sensory neurons was isolated and purified by DEAE Sephacel ion exchange chromatography and high performance liquid chromatography. A primary analysis of its secondary structure by circular dichroism, and its neurotrophic effects were investigated using the model of dorsal root ganglia(DRG) cultured in vitro . Results The molecular weight and isoelectric point of the protein were 33 1 kDa and 5 52, respectively. Its circular dichroism showed that there were 20 8% α helix, 54 8% β sheet, 7 3% turn, and 17 1% random coil in its secondary structure. Biological experiments showed that the protein could promote the neurite outgrowth of DRG. Conclusion A specific protein in spinal sensory tissue with molecular weight of 33 1 kDa has been purified. There is mainly β sheet in the secondary structure of the protein. And the protein has neurotrophic effects in the model of DRG.目的　纯化大鼠脊神经感觉纤维 33.1kDa特异蛋白 (SSP 33.1) ,并分析其二级结构及其神经营养活性。方法　采用双向电泳技术比较了大鼠脊髓背根神经纤维和腹根神经纤维中蛋白质表达差异 ,用DEAE Sephacel阴离子交换层析和高效液相凝胶过滤的方法纯化了其中一种蛋白质 ,并通过圆二色谱初步分析了该蛋白质的二级结构 ;又通过经典的神经营养活性的模型 -鸡胚背根节 (DRG)体外培养模型 ,测定该蛋白的生物学活性。结果　纯化的蛋白质分子量为 33.1,等电点为 5 .5 2 ,其二级结构中α螺旋占 2 0 .8% ,β片层占 5 4 .8% ,转角占 7.3% ,无规卷曲占 17.1%。体外实验表明该蛋白质能促进鸡胚背根神经节突起的生长。结论　纯化了大鼠感觉神经 33.1kDa特异蛋白 ,该蛋白质二级结构以 β片层为主 。

关 键 词：spinal sensory specific 33  1 kDa protein two dimensional electrophoresis high performance liquid chromatography circular dichroism neurotrophic effect 

分 类 号：R341[医药卫生—基础医学]