葡萄叶片饲喂的^(14)C-水杨酸对高温胁迫的应激反应  

Response of ^(14)C-salicylic Acid to Heat Stress After Being Fed to Leaves of Grape Seedlings

作　　者：刘悦萍[1] 黄卫东[1] 王利军[1] 

机构地区：[1]中国农业大学园艺学院,北京100094

出　　处：《中国农业科学》2003年第6期685-690,共6页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助项目 ( 3 0 0 70 5 3 1)

摘　　要：采用具 8片功能叶的葡萄幼苗 ,摘除第 1、2、8片叶 ,在中部第 5片叶 (L 5 )上饲喂14 C SA ,分别对L 5对侧上方叶 (L 4 )和下方叶 (L 6 )进行 4 0± 0 .5℃高温处理。结果发现 ,高温处理增加了饲喂叶14 C SA的向外运转量 ,改变了14 C SA在各器官的分配比例。高温处理L 4或L 6叶后 ,处理叶片中的14 C SA积累量至少为对照的 3倍以上 ,植株其余叶片 (L 3或L 7)中的14 C SA积累量则低于对照 ;14 C SA向茎段的分配量与高温处理叶的位置相关。另外 ,高温改变了14 C SA向饲喂叶上侧和下侧的分配比例 ,最终均表现为向高温处理部位一侧的运输量增加。直接用仪器测得的14 C比放射强度至少有 70 %以上是14 C SA ,表明SA在运转中的代谢不多。All leaves of grape ( Vitis vinifera×V. labrussa L. cv. Jingxiu) seedlings were removed except the 3rd, 4th, 5th, 6th, and 7th ones. The 5th leaf was fed with 14 C SA, and the 4th or 6th leaf was at a high temperature of 40±0.5℃. There were more 14 C SA being transported out from the 5th leaf at high temperature than at normal temperature and changed the ratio of 14 C SA distribution in each organ. The accumulation of 14 C SA in 4th or 6th leaf treated at high temperature was at least three times as much as that at normal temperature. The distribution of 14 C SA in other leaves (the 3rd and 7th leaves) decreased; more 14 C SA accumulated in stems which adjacent to the 4th or 6th leaf treated at high temperature. In addition, more 14 C SA transported upwards or downwards while one of its leaf treated at high temperature. The ratio of radioactivity of 14 C assayed after SA being extracted to direct assay with apparatus was more than 70%.

关 键 词：葡萄叶片 14^C-水杨酸 高温胁迫 应激反应 植物抗病力 

分 类 号：Q945[生物学—植物学]