检测尿MCP-1诊断移植肾急性排斥反应的研究  

Study on the diagnosis of acute rejection upon the method of ELISA for detecting the MCP-1 in urine of renal transplant recipient

作　　者：曹伟[1] 李黔生[1] 吴玉章[2] 吴军[3] 吕凤林[4] 霍文谦[1] 颜加强[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所泌尿外科洗肾移植中心,重庆400042 [2]第三军医大学全军免疫学研究所,重庆400038 [3]第三军医大学西南医院烧伤研究所,重庆400038 [4]第三军医大学大坪医院野战外科研究所第一研究室,重庆400042

出　　处：《免疫学杂志》2003年第4期307-308,311,共3页Immunological Journal

摘　　要：目的　动态检测肾移植受者尿液单核细胞趋化性肽 1(MCP 1)的含量 ,了解其变化规律 ,探讨其在肾移植急性排斥反应中的意义。方法　应用酶联免疫吸附法 (ELISA) ,测定了 74例肾移植受者和 10例无肾脏疾病患者尿液单核细胞趋化性肽 1(MCP 1)的含量。结果　肾功能稳定的肾移植受者尿液MCP 1水平 (412± 2 5 )ng mL ,正常对照组为 (40 7+12 )ng mL ,二者无明显差异 (P >0 .0 5 )。发生急性排斥反应时尿液MCP 1水平为 (1193+5 6 )ng mL ,明显高于正常对照和肾功能稳定组(P <0 .0 1)。经抗排斥治疗后 ,对治疗有反应的受者尿液MCP 1浓度明显降低。结论　尿液MCP 1水平与肾移植急性排斥反应有密切关系 ;检测尿液MCPObjective Monocyte chemotactic peptide-1(MCP-1)is a specific chemotactic and activating factor for monocytes in acute renal transplant rejection. The present study is aimed at evaluating urinary MCP-1 excretion levels in renal transplant recipients.Methods Urine levels of monocyte chemotactic peptide-1(MCP-1)in 74 cases of renal transplant recipients and 10 cases of non-renal-disease subjects were measured with a ELISA method.Results The results showed that the MCP-1 urine levels are strikingly higher in recipients with acute rejection than in clinically stable ones (P<0.01) and normal subjects (P<0.01). There was a significant reduction in MCP-1 urine levels in patients with acute rejection, who responded to the anti-rejection treatment.Conclusions The urinary MCP-1 excretion correlates with acute rejection. An increase in urinary MCP-1 excretion may represent an early signal of ongoing acute renal rejection. MCP-1 may be a useful marker for early diagnosis and responsiveness after anti-rejection treatment.

关 键 词：检测 尿 MCP-1 诊断 移植肾 急性排斥反应 研究 

分 类 号：R446.61[医药卫生—诊断学]