支气管哮喘患者外周血单核细胞Th1/Th2型细胞因子水平的检测及其临床意义  

Th1-and Th2-type cytokine levels from peripheral blood mononuclear cells in patients with asthma

作　　者：岑慧[1] 郭兰萍[1] 黎东明[1] 张卫珍[1] 李文[1] 梁标[1] 

机构地区：[1]广东医学院附属医院呼吸内科,广东湛江524001

出　　处：《广东医学院学报》2003年第3期208-209,共2页Journal of Guangdong Medical College

摘　　要：目的 :观察支气管哮喘患者在发病期外周血中的Th 1/Th 2型细胞因子水平的变化。方法 :采用双抗体夹心ELISA方法分别对哮喘患者发作期组 (n =15 )、缓解期组 (n =15 )及正常人对照组 (n =2 0 )外周血单核细胞 (PBMC)产生的Th 1/Th 2型细胞因子 (IL 2、IL 4、IL 10 )进行了测定。结果 :哮喘急性发作期患者PBMC产生的IL 2水平较正常对照组明显下降 (P <0 .0 5 ) ,而IL 4、IL 10水平均增高 (P <0 .0 5 ) ,缓解期恢复至正常水平。结论 :哮喘发病过程中Th 1/Th 2型细胞因子处于失衡状态 。Objective:To observe the change of Th1 and Th2 type cytokine levels from peripheral blood mononuclear cells(PBMCs) in patients with asthma.Methods:IL 2, 4 and 10 contents of PBMCs in patients with asthma and normal controls were determined by ELISA.Results:Compared with control group,IL 2 level was signnificantly decreased,and IL 4 and 10 concentrations were remarkably increased in patients with acute stage of asthma( P< 0.05);whereas they were all returned to normal in convalescent stage.Conclusion:There could be an imbalance of Th1 and Th2 type cytokine profiles in the pathogenesis of asthma. [

关 键 词：支气管哮喘 外周血 单核细胞 TH1/TH2型 细胞因子 检测 

分 类 号：R562.25[医药卫生—呼吸系统]