HPLC测定氧化苦参碱氯化钠注射液含量及有关物质  

Determination of Oxymatrine and Related Substances In Oxymatrine NaCI Injection by HPLC

作　　者：辛爱学 曲福军[2] 孙华[2] 张一飞[2] 

机构地区：[1]武警黄金一总队医院药剂科,150086 [2]哈尔滨医科大学附属第二医院临床药学药物研究所

出　　处：《黑龙江医药》2003年第4期251-253,共3页Heilongjiang Medicine journal

摘　　要：目的:建立氧化苦参碱氯化钠注射液含量及有关物质测定方法。方法:反相高效液相色谱法测定氧化苦参碱的含量及有关物质,外标法定量,分析几种色谱条件下的分离效果,确定最佳分离条件。结果:氧化苦参碱的测定线性范围0.032～0.8mg/ml,r=0.9998,平均回收率100.5%,RSD0.6%(n=9),精密度 RSD0.6%(n=9)。结论:该方法适合于氧化苦参碱氯化钠注射液中氧化苦参碱的含量测定及有关物质检查。Objectives:To establish RP-HPLC method for the determination of oxymatrine and related substances in oxymatrine NaCl injection.Methods:To determination of oxymatrine and related substances in oxymatrine NaCl injection by RP-HPLC with external standard method.We compared with several separation effects under different chromatography conditions. And a best chromatography condition was found.Results:The best separation was obstained.The linear range was 0.032-0.08mg/ml for oxymation r=0.9998.The average recovery of oxyma- trine was 100.5% RSD0.6%(n=9)pre

关 键 词：HPLC测定 氧化苦参碱氯化钠注射液 含量 外标法定量 

分 类 号：R927.2[医药卫生—药学]