非靶位基因突变及外膜通透性的改变对大肠杆菌喹诺酮耐药和多重耐药的影响  

Impact of non-target gene mutations and reduced permeability of outer membrane on quinolone resistance and multiple antibiotic resistance in Escherichia coli

作　　者：赵瑞华[1] 舒明星[1] 陈淑贞[1] 

机构地区：[1]中南大学湘雅医学院微生物学教研室,长沙410078

出　　处：《湖南医科大学学报》2003年第3期263-265,共3页Bulletin of Hunan Medical University

摘　　要：目的 :探讨非靶位基因突变及外膜通透性改变对大肠杆菌喹诺酮类耐药和多重耐药的影响。方法 :PCR扩增大肠杆菌marOR区并进行DNA测序分析 ,提取外膜蛋白进行组成型分析。结果 :在R4 1 0菌株中 ,存在两处新位点的突变 :1 870 (T→C ,Val→Ala)和 1 879(A→C ,terminator→Ser)。 3株对喹诺酮类药物高耐且同时多耐的菌株均存在OmpF缺失。结论 :多重耐药基因marOR区内的突变可能导致MarA表达增加和外膜蛋白组成型改变(OmpF缺失 ) 。Objective To investigate the impact of non target gene mutations and reduced permeability of the outer membrane on quinolone resistance and multiple antibiotic resistance(mar) in Escherichia coli . Methods Several high level quinolone resistant isolates whose strains were accompanied by concurrent resistant to several antibiotics were selected. The PCR products of marOR region were sequenced to detect the possible gene changes and then the strains' outer membrane proteins (Omps)were extracted to analyze on the constitutive profiles.Results We found two site mutations in R410 isolate:1 870(T→C,Val→Ala)and 1 879(A→C,terminator→Ser). The OmpF deficiency was found in all 3 resistant strains. Conclusion The increased expression of MarA induced by gene mutations in marOR and the constitutive profile changes of the Omps (OmpF deficient strains) may play some role in the quinolone resistance and multiple antibiotic resistance of Escherichia coli .

关 键 词：非靶位基因突变 外膜通透性 大肠杆菌 喹诺酮 耐药 多重耐药 影响 细菌外膜蛋白 

分 类 号：R96[医药卫生—药理学]