反相高效液相色谱法测定肾炎汤中淫羊藿苷的含量  

Determination of Icariine in Shenyantang by RP-HPLC

作　　者：吕永宁[1] 王虎[1] 童大刚[1] 徐楚鸿[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院,湖北武汉430022

出　　处：《中国医院药学杂志》2003年第7期411-412,共2页Chinese Journal of Hospital Pharmacy

摘　　要：目的 :反相高效液相色谱法 (RP HPLC )法测定肾炎汤中淫羊藿苷的含量。方法 :HypersilC18柱 ,流动相为乙腈 水( 2 7∶73) ,在 2 70nm波长处检测。结果 :淫羊藿苷的线性范围为 0 .0 7～ 0 .6 3μg ,r =0 .9999,平均加样回收率为 99%。结论 :方法简便 ,精密度和稳定性好 ,适用于测定肾炎汤中淫羊藿苷的含量。OBJECTIVE To establish an HPLC method to determine the content of icariine in Shenyantang .METHODS HYPERSIL C 18 column was used.The mobile phase consisted of acetonitrile water (27∶73).The detection wavelength was 270 nm. RESULTS The linear range was 0.07 ～ 0.63 μg, r = 0.9999 .The average recovery of icariine was 99%.CONCLUSIONS This RP HPLC method is convenient,accurate, stable and is suitable for the content determination of icariine in Shenyantang .

关 键 词：淫羊藿苷 肾炎汤 反相高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]