促性腺素释放激素主动免疫对公、母猪生殖机能的影响(英文)  

Effect of Active Immunization Against GnRH on Reproductive Function in Boars and Gilts

作　　者：黄夺先[1] 赵伟[1] 孙益兴 朱建辉[1] 吴美珍[1] 

机构地区：[1]江苏省农业科学院畜牧兽医研究所,南京210014

出　　处：《江苏农业学报》1992年第2期31-35,共5页Jiangsu Journal of Agricultural Sciences

摘　　要：六头公猪和六头母猪在断奶时用促性腺素释放激素(GnRH)主动免疫,观测内源GnRH被免疫中和后所产生的内分泌和生殖机能的变化.注射GnRH与牛血清蛋白(BSA)的偶联物二次,所有受试动物均能产生GnRH的抗体.GnRH主动免疫后性活动受到阻抑,同时降低血浆睾酮浓度(P<0.01)和阻碍精子发生,睾丸、附睾、卵巢和子宫重也明显减少(P<0.01).结果表明公、母猪接种GnRH-BSA能诱发免疫反应,中和内源GnRH的生物活性,且抑制睾酮、孕酮和17β-雌二醇的合成,从而导致性器官发育受阻,达到免疫去势的目的.Six boars and 6 gilts at weaning were actively im-munized against gonadotropin releasing hormone (GnRH) to observe changes in endocrine and reproduc-tive f unction associated with immunoneutralization of en-dogenous GnRH. Two injections of GnRH conjugated to bovine serum ablumin (BSA) induced production of anti-bodies against GnRH in all immunized boars and gilts. Active immunization against GnRH reduced plasma con-centration of testosterone (P< 0.01), paired testes weight (P<0. 01), paired epididymis weight (P< 0. 01 ), weights of ovary and uterus (P<0. 01) and seminiferous tubule diameter (P<0. 01) compared with controls. These results indicate that the immune response induced by inoculation of GnRH-BSA in boars and gilts neutralized the biological activity of endogenous GnRH and inhibited the synthesis of testosterone, progesterone and estradiol-17 ,resulting in blocking the development of sexual organs.

关 键 词：母猪 促性腺素 释放激素 免疫 猪 

分 类 号：S828[农业科学—畜牧学]