涝渍逆境下玉米叶片各细胞器超氧物歧化酶活性的变化及6-BA的保护作用  

Superoxide Dismutase Activity in Leaf-Cell Organelles and Protective Role of 6-BA against Damage in Corn under Waterlogging Stress

作　　者：李建坤[1] 汪宗立 

机构地区：[1]江苏省农业科学院农业生物遗传生理研究所,南京210014

出　　处：《江苏农业学报》1992年第3期20-24,共5页Jiangsu Journal of Agricultural Sciences

基　　金：农业部"七五"课题"05-01-02";国家自然科学基金"3860644"项目的资助

摘　　要：玉米叶片超氧物歧化酶(SOD)活性在各细胞器中的分布以细胞溶质部分最高,约占总活性的85％,叶绿体和线粒体部分各占13％和2％。土壤淹水使各细胞器SOD活性下降,叶绿体是受影响最敏感的亚细胞组分。6-BA喷株能明显促进受涝玉米叶片各细胞器中SOD活性升高,阻止叶片细胞的膜脂过氧化,从而增强对涝渍伤害的抗性。The proportions of superoxide dismutase (SOD) activity in corn leaf-cell organelles under normal growth condition and changes in the SOD activity as affected by waterlogging stress were investigated. The effect of 6-benzylademine (6-BA) on the induced SOD activity in cytosol, chloroplasts and mitochondria of waterlogged corn leaves was also studied. The results indicated that-about 85% of the total SOD activity in corn leaves was present in cytosol (11,000Xg supernatant fraction). The rest was in chloroplasts (1000Xg precipitate fraction) and mitochondria (11,00Xg precipitate fraction), approximately 13% and 2% of the total SOD activity respectively. Waterlogging caused an obvious decrease in SOD activity in all organelles examined. The SOD sensitivity to waterlogging varied with organelles. The chloroplast was the most sensitive one. It is interesting to note that applications of 6-BA (10ppm) just before waterlogging and 12 h later led to an apparent increase in SOD activity in all cell components examined, and to a marked decrease in membrane lipid peroxidation in the leaves. Thus, 6-BA enhanced the resistance of corn plants to waterlogging.

关 键 词：玉米 涝渍逆境 超氧化物歧化酶 6-BA 

分 类 号：S513.01[农业科学—作物学]