补肾活血方对离体胎鼠颅骨成骨细胞增殖的影响  

Effects of Chinese medicine Bushenhuoxue on the proliferation of fetal rat calvarial osteoblast in vitro

作　　者：汤耿民[1] 沈霖[2] 肖明英[1] 

机构地区：[1]湖北省中医药研究院,湖北武汉430074 [2]华中科技大学同济医学院附属协和医院

出　　处：《中国骨伤》2003年第4期211-212,共2页China Journal of Orthopaedics and Traumatology

基　　金：湖北省卫生科研基金(W9721);湖北省自然科学基金(99J136)

摘　　要：目的 探讨补肾活血法对成骨细胞代谢的影响。方法 胎鼠颅骨成骨细胞培养于含有0,10,100,1000,5 000μg·ml^(-1)5种浓度的补肾活血方药液培养基中72h后,分别采用噻唑篮比色法和胸腺嘧啶核苷渗入法检测成骨细胞增殖、分化情况,并与具有促细胞增殖作用的成纤维细胞生长因子作对照。结果 补肾活血方药液以剂量依赖方式促进成骨细胞的增殖,与对照组相比,100 g·d-‘药液即可促进成骨细胞增殖,并在1000μg·ml^(-1)浓度时达到最大效应(P<0.05,P<0.01)。结论 补肾活血方具有促进骨形成作用。Objective To investigate the effects of Bushenghuoxue on osteoblastic cell metabolism Methods Fetal rat calvarial osteoblastic cell were cultured in the medium containing Bushenghuoxue with five different concentrations,0,10,100,1 000,and 5 000μg·ml-1 ,and incubated for 72 hours.Calorimetric MMT,3H-thymidine incorporation were used to determine the condition of cell proliferation and differentiation in vitro. Results Bushenghuoxue with 100 μg·ml-1 might promoted the proliferation of osteoblastic cells. Its effects was concentration-dependent. 1000 μg·ml-1 may reach the highest effect. Conclusion Bushenghuoxue can promote the bone formation.

关 键 词：补肾活血方 离体胎鼠 颅骨 成骨细胞 影响 细胞增殖 实验 

分 类 号：R285.5[医药卫生—中药学]