家兔骨折愈合过程中软骨细胞凋亡的透射电镜观察  

Transmission electron microscopy for the chondrocyte apoptosis in rabbit's fracture healing

作　　者：严蕊琳[1] 马时荣[1] 王桂华[2] 王祖武[3] 

机构地区：[1]嘉兴学院医学院,浙江嘉兴314001 [2]嘉兴医学院附属第二医院 [3]杭州师范学院医学院

出　　处：《中国骨伤》2003年第4期217-219,共3页China Journal of Orthopaedics and Traumatology

摘　　要：目的 观察骨折愈合中软骨细胞在软骨化骨时的死亡方式及软骨细胞的其它可能结局。方法 采用家兔左桡骨标准骨折,在软骨骨痂产生的不同时期、不同部位取材,常规制作透射电镜观察标本。结果 ①软骨细胞生长、消亡过程可分为3个阶段:成软骨细胞、肥大软骨细胞、凋亡软骨细胞。软骨细胞凋亡表现为核固缩和凋亡小体形成,但细胞器不被破坏。最后,软骨细胞崩解,凋亡小体散落于软骨基质中。②软骨化骨过程中只是部分软骨细胞凋亡;另一部分软骨细胞并不出现凋亡,直接转变为骨细胞。结论 ①骨折愈合时发生的软骨化骨,是机体通过激活软骨细胞死亡程序,以细胞凋亡达到新老细胞交替,实现化骨。②由肥大软骨细胞组成的软骨骨痂,能直接转变为编织骨并骨化,支持软骨细胞能直接转变为骨细胞的理论。Objective To observe the dead manner and other possible outcomes of chondrocytes in the healing process of fracture Methods The standardized fracture of left radial bone was created in rabbits. The cartilage callus at the fracture site were taken at the different healing time and different position,and observed under the transmission electron microscope. Results The results of the study indicated that there were three stages of chondrocyte changes at the healing process: chondroblasts, hypertrophic chondrocytes and apoptotic chondrocytes. The apoptosis of chondrocytes showed nucleus shrink and apoptotic bodies,but no rupture of or-ganelles. Finally,the apoptotic chondrocytes broke down and many apoptotic bodies were in cartilage matrix. Also it was found that not all of chondrocytes appeared apoptosis in the endochondrial ossification process. The chondrocytes without apoptosis might directly transform into osteocytes. Conclusion ① In fracture healing process, the endochondrial ossification was mediated by chondrocyte apoptosis. ②The cartilage callus made of hypertrophic chondrocytes can directly transform into the woven bones.

关 键 词：家兔 骨折愈合 软骨细胞 细胞凋亡 透射电镜 电子显微镜检查 

分 类 号：R683[医药卫生—骨科学]